An imaging device based on fluorescence for in vivo detection of oral cancer has been developed. Images collected from cancerous patients and normal volunteers have shown a clear difference in fluorescence intensity
In this study a hand held probe has been automated using a motorized stage and LabVIEW based control and acquisition system for in-vitro cervical precancer diagnosis. The stage movement is controlled by two motors for vertical and horizontal movement while a third motor is utilized for changing the polarization state. The data acquisition and storing was thus performed by moving the probe to a specific site, acquiring polarized fluorescence and elastic scattering signals and creating a user friendly graphical user interface(GUI) panel to suit our needs. Another GUI in MATLAB has also been developed for the real time analysis of data captured through the automatic acquisition system. Three windows were created inside the GUI to display calculated intrinsic fluorescence, for statistical analysis and classification. Principal component analysis was applied to extract the features and classification was done by Mahalanobis distance based algorithm. The classification results have been presented of different grades of cervical cancer
An in-house fabricated portable device has been tested to detect cervical precancer through the intrinsic fluorescence from human cervix of the whole uterus in a clinical setting. A previously validated technique based on simultaneously acquired polarized fluorescence and polarized elastic scattering spectra from a turbid medium is used to extract the intrinsic fluorescence. Using a diode laser at 405 nm, intrinsic fluorescence of flavin adenine dinucleotide, which is the dominant fluorophore and other contributing fluorophores in the epithelium of cervical tissue, has been extracted. Different grades of cervical precancer (cervical intraepithelial neoplasia; CIN) have been discriminated using principal component analysis-based Mahalanobis distance and linear discriminant analysis. Normal, CIN I and CIN II samples have been discriminated from one another with high sensitivity and specificity at 95% confidence level. This ex vivo study with cervix of whole uterus samples immediately after hysterectomy in a clinical environment indicates that the in-house fabricated portable device has the potential to be used as a screening tool for in vivo precancer detection using intrinsic fluorescence.
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