Pteridines and its derivatives are considered as important cofactors participating in cellular metabolism. Studies reported that, the distribution of pteridines and its derivatives may change when monocytes and macrophages are activated under interferon- γ stimulus by cancer. Also, there is a significant variation in the concentration and conformation of pteridines under different pathological conditions. It has been reported that during the transformation of normal cells into neoplasm, the metabolic end products of the cancer cells are released into the blood, thereby changing the components and contents of the biological molecules and their local environment. In this regard, the present study is aimed to characterize pteridines and its derivatives in the blood plasma of normal subjects and patients with confirmed oral cancer using fluorescence and Raman spectroscopy. Observed Fluorescence & Raman spectral characteristics of samples and subsequent discriminant analysis predicts that 75 % of the original and cross validated groups are correctly classified.
Urine is one of the diagnostically important bio fluids, as it has many metabolites and some of them are native fluorophores. Riboflavin and its cofactors FMN and FAD which act as electron carriers participates in a diversity of redox reactions central to human metabolism. It has been reported that riboflavin plays a prominent role in progression of various cancers. It is well documented that, the fluorophore flavins that is not bound to proteins in the plasma is filtered by glomerulus and excreted in urine. Fluorescence spectroscopy has been considered as a promising tool to characterize the riboflavin present in the urine. The overall spectral data at 450 nm excitation were subjected to principal components based linear discriminant analysis. As a result, 100 % of the normal subjects and 90% of the cervical cancer subjects were correctly classified which shows that there exists significant difference between them.
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