Recent developments in optogenetics allow for quick and minimally invasive methods of mapping functional brain circuits in animal models. DeepLabCut (DLC), a toolbox for markerless pose estimation, offers the ability to track features in three-dimensions. We demonstrate a hybrid method utilizing DLC and light-based, optogenetic motor mapping to concurrently localize motor representations of multiple limbs in mice. Our results suggest that behaviorally-relevant, motor movements involving multiple limbs reside in overlapping cortical representations of each limb. Applications of this technique include characterizing recovery of finer, articulated movements of affected limbs after stroke, or mapping brain network activity during naturalistic behavior.
Recent developments in optogenetics allow for quick and minimally-invasive methods of studying functional brain organization in animal models. DeepLabCut (DLC), a toolbox for markerless pose estimation, offers the ability to track user-defined features in 3-dimensions with human level accuracy. We demonstrate a hybrid method utilizing DLC and optogenetic motor mapping to localize the movements of multiple modalities to the mouse cortex. We outline a pipeline to map and characterize multiple motor representations in anesthetized and awake mice. Furthermore, we identify behaviorally-relevant motor movements of multiple limbs reside in overlapping cortical representations of the respective limbs.
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