The recently announced confirmation of a global ocean beneath the icy outer shell of the Saturnian moon Enceladus provides strong support for cometary panspermia. Recent discoveries have shown that cyanobacteria, diatoms and other photosynthetic microphytoplankton live in the deep, dark bathysphere of the terrestrial oceans. Evidence for liquid water regimes that might harbour life and organics on other icy moons, comets and Pluto adds credence to the concept of a single connected microbial biosphere in the solar system. These discoveries provide additional support for the possibility that life may be widely distributed throughout the distant regions of the Solar System and the validity of the hypothesis of Panspermia.
The emerging consensus that comets carry the biochemical seeds of life coincides with the first step that was reached as early as 1977 in the historical development of the Hoyle-Wickramasinghe theory of cosmic life. To mark the centenary of the birth of Sir Fred Hoyle on 24 June 2015 this brief article retraces early developments that essentially heralded the new science of astrobiology.
The question of the contamination of meteorites by modern environmental microorganisms is an issue that has been raised since evidence for biological remains in carbonaceous meteorites was first published in the early 1960's.1-3 The contamination hypothesis has been raised for recent fossils of diatoms and filamentous cyanobacteria found embedded in the stones even though the nitrogen content of the fossils was below the 0.5% detection limit for Energy Dispersive X-ray Spectroscopy (EDS) of the Field Emission Scanning Electron Microscope. All modern biological contaminants should have nitrogen content in the detectable range of 2% to 20% indicating the remains are ancient fossils rather than living or Holocene cells. In our work, the possibility that extremophilic bacteria from our lab collection might be able to metabolize organic matter in the studied meteorites was tested. The potential toxic or inhibitory growth effects were also checked for different anaerobic cultures. UV exposed meteorite samples with consequent sterile extraction of the internal part were subjected to anaerobic cultivation techniques. As a result, eight anaerobic strains were isolated from internal and exterior parts of the studied meteorites. Preliminary results of their morphology, cytology, physiology, and molecular (16SrRNA sequencing) studies are presented and discussed in this article.
We report on the physical, chemical and mineral properties of a series of stone fragments recovered from the North Central Province of Sri Lanka following a witnessed fireball event on 29 December 2012. The stones exhibit highly porous poikilitic textures comprising of isotropic silica-rich/plagioclase-like hosts. Inclusions range in size and shape from mm-sized to smaller subangular grains frequently more fractured than the surrounding host and include ilmenite, olivine (fayalitic), quartz and accessory zircon. Bulk mineral compositions include accessory cristobalite, hercynite, anorthite, wuestite, albite, anorthoclase and the high pressure olivine polymorph wadsleyite, suggesting previous endurance of a shock pressure of ~20 GPa. Further evidence of shock is confirmed by the conversion of all plagioclase to maskelynite. Here the infrared absorption spectra in the region 580 cm-1 to 380 cm-1 due to the Si-O-Si or Si-O-Al absorption band shows a partial shift in the peak at 380 cm-1 towards 480 cm-1 indicating an intermediate position between crystalline and amorphous phase. Host matrix chemical compositions vary between samples, but all are rich in SiO2. Silica-rich melts display a heterogeneous K-enrichment comparable to that reported in a range of non-terrestrial material from rare iron meteorites to LL chondritic breccias and Lunar granites. Bulk chemical compositions of plagioclase-like samples are comparable to reported data e.g. Miller Ranger 05035 (Lunar), while Si-rich samples accord well with mafic and felsic glasses reported in NWA 1664 (Howardite) as well as data for fusion crust present in a variety of meteoritic samples. Triple oxygen isotope results show Δ17O = -0.335 with δ18O (‰ rel. SMOW) values of 17.816 ± 0.100 and compare well with those of known CI chondrites and are within the range of CI-like (Meta-C) chondrites. Rare earth elemental abundances show a profound Europium anomaly of between 0.7 and 0.9 ppm while CI normalized REE patterns accord well with those of high potassium and high aluminium glasses found in lunar and 4 Vesta samples. Twoelement discrimination maps of FeO vs SiO2, FeO vs TiO2, FeO vs Al2O3 and FeO vs Na2O similarly match those of impact glasses present in lunar samples and remain within relatively close proximity of the KREEP component. Iridium levels of between 1-7ppm, approximately 104 times that of terrestrial crustal rocks, were detected in all samples.
On December 29, 2012, a bright yellow and green fireball was observed to disintegrate over the Polonnaruwa District of North Central, Sri Lanka. Many low density, black stones were recovered soon after the observed fall from rice paddy fields near the villages of Aralaganwila and Dimbulagala. These stones were initially studied by optical microscopy methods at the Medical Research Institute in Colombo, Sri Lanka. Soon thereafter, samples were sent to the UK and to the United States. More extensive Field Emission Scanning Electron Microscopy studies were then carried out at Cardiff University and the NASA/Marshall Space Flight Center. The physico-chemical properties, elemental abundances, mineralogy and stable isotope data clearly indicate that these stones are non-terrestrial. Freshly fractured interior surfaces of the black stones have also been observed to contain the remains of fossilized diatom. Many of the diatom frustules are clearly embedded in the meteorite rock matrix and exhibit nitrogen levels below the EDX detection limits. Some of the fossil diatoms are araphid marine pennates and planktonic forms that are inconsistent with conditions associated with rice paddy fields. These observations indicate the fossilized diatoms are indigenous to the meteorites rather than post-arrival biological contaminants. The carbon content and mineralogy suggests that these stones may represent a previously ungrouped clan of carbonaceous meteorites. The extremely low density (~0.6) of the stones and their observed mineralogy was inconsistent with known terrestrial rocks (e.g., pumice, diatomite and fulgurites). The minerals detected suggest that the parent body of the Polonnaruwa stones may have been the nucleus of a comet. These observations are interpreted as supporting the Hoyle-Wickramasinghe Panspermia hypothesis and the hypothesis that diatoms and other microorganisms might be capable of living and growing in water ice and brines in comets.
Biological entities were isolated at a height of between 22-27 km in the stratosphere. Sampling of this region was carried out in the UK in July 2013 using a relatively simple low-cost balloon-borne sampler carrying aseptically clean scanning electron microscope stubs onto which aerosols were directly captured. The entities varied from a presumptive colony of ultra-small bacteria to two unusual individual organisms - part of a diatom frustule and a 200 micron-sized particle mass interlaced with biological filaments. Biological entities of this nature have not previously been reported occurring in the stratosphere; their likely origin is discussed and we provide arguments to support our view that such biological entities may have arrived from space. The new data gives strong confirmation of the Hoyle-Wickramasinghe theory of cometary panspermia.
Recent studies of comets and cometary dust have confirmed the presence of biologically relevant organic molecules
along with clay minerals and water ice. It is also now well established by deuterium/hydrogen ratios that the CI1
carbonaceous meteorites contain indigenous extraterrestrial water. The evidence of extensive aqueous alteration of the
minerals in these meteorites led to the hypothesis that water-bearing asteroids or comets represent the parent bodies of
the CI1 (and perhaps CM2) carbonaceous meteorites. These meteorites have also been shown to possess a diverse array
of complex organics and chiral and morphological biomarkers. Stable isotope studies by numerous independent
investigators have conclusively established that the complex organics found in these meteorites are both indigenous and
extraterrestrial in nature. Although the origin of these organics is still unknown, some researchers have suggested that
they originated by unknown abiotic mechanisms and may have played a role in the delivery of chiral biomolecules and
the origin of life on Early Earth.
Preliminary SEM/EDAX studies of the Tissint meteorite shows projections of interior spherical globules rich in C and O. Such concentrations of carbonaceous material in a matrix of mineral grains pose a mystery. These structures are consistent with remnants of biological structures.
Recent studies of comets and cometary dust have confirmed the presence of biologically relevant organic molecules
along with clay minerals and water ice. It is also now well established by deuterium/hydrogen ratios that the CI1
carbonaceous meteorites contain indigenous extraterrestrial water. The evidence of extensive aqueous alteration of the
minerals in these meteorites led to the hypothesis that water-bearing asteroids or comets represent the parent bodies of
the CI1 (and perhaps CM2) carbonaceous meteorites. These meteorites have also been shown to possess a diverse array
of complex organics and chiral and morphological biomarkers. Stable isotope studies by numerous independent
investigators have conclusively established that the complex organics found in these meteorites are both indigenous and
extraterrestrial in nature. Although the origin of these organics is still unknown, some researchers have suggested that
they originated by unknown abiotic mechanisms and may have played a role in the delivery of chiral biomolecules and
the origin of life on Early Earth.
In this paper we review these results and investigate the thermal history of comets. We show that permanent as well as
transient domains of liquid water can be maintained on a comet under a plausible set of assumptions. With each
perihelion passage of a comet volatiles are preferentially released, and during millions of such passages the comet could
shed crustal debris that may survive transit through the Earth's atmosphere as a carbonaceous meteorite. We review the
current state of knowledge of comets and carbonaceous meteorites. We also present the results of recent studies on the
long-term viability of terrestrial ice-microbiota encased in ancient glacial ice and permafrost. We suggest that the
conditions which have been observed to prevail on many comets do not preclude either survivability (or even the active
metabolism and growth) of many types of eukaryotic and prokaryotic microbial extremophiles-including algae,
cyanobacteria, bacteria and archaea. It is argued that the chemical and morphological biomarkers detected on comets and
carbonaceous meteorites can be explained by ancient microbial activity without the need to invoke unknown abiotic
production mechanisms.
Three decades ago the first convincing evidence of microbial fossils in carbonaceous chondrites was discovered and
reported by Hans Dieter Pflug and his collaborators. In addition to morphology, other data, notably laser mass
spectroscopy, confirmed the identification of such structures as putative bacterial fossils. Balloon-borne cryosampling
of the stratosphere enables recovery of fragile cometary dust aggregates with their structure and carbonaceous matter
largely intact. SEM studies of texture and morphology of particles in the Cardiff collection, together with EDX
identifications, show two main types of putative bio-fossils - firstly organic-walled hollow spheres around 10μm across,
secondly siliceous diatom skeletons similar to those found in carbonaceous chondrites and terrestrial sedimentary rocks
and termed 'acritarchs'. Since carbonaceous chondrites (particularly Type 1 chondrites) are thought to be extinct
comets the data reviewed in this article provide strong support for theories of cometary panspermia.
We explore the conditions prevailing in primordial planets in the framework of the HGD cosmologies as discussed by
Gibson and Schild. The initial stages of condensation of planet-mass gas clouds is set at 300,000 yr (0.3My) following
the onset of plasma instabilities when ambient temperatures were >1000K. Eventual collapse of the cloud into a solid
structure, dominated by water-ice and organics takes place against the background of an expanding universe with
declining ambient temperatures. Isothermal free fall collapse occurs initially via quasi equilibrium polytropes until
opacity sets in due to molecule and dust formation. The contracting cooling cloud is a venue for molecule formation and
the sequential condensation of solid particles, starting from mineral grains at high temperatures to ice particles at lower
temperatures, Water-ice becomes thermodynamically stable between 7 and 15 My after the initial onset of collapse, and
contraction to form a solid icy core begins shortly thereafter. The icy planet core, which includes a fraction of
radioactive nuclides, 26Al and 60Fe, melts through interior heating. We show, using heat conduction calculations, that the
interior domains remain liquid for tens of My for 300km and 1000km objects, but not for 30 or 50km objects. Initially
planets are separated by relatively short distances, measured in tens to hundreds of AU, because of the high density of
the early universe. Thus exchanges of materials, organic molecules and evolving templates could readily occur
providing optimal conditions for an initial origin of life. The condensation of solid molecular hydrogen as an extended
outer crust takes place much later in the collapse history of the protoplanet. When the object has shrunk to several times
the radius of Jupiter, the hydrogen partial pressure exceeds the saturation vapour pressure of solid hydrogen at the
ambient temperature and condensation occurs.
We have shown that the red cells found in the Red Rain (which fell on Kerala, India, in 2001) survive and grow after
incubation for periods of up to two hours at 121°C . Under these conditions daughter cells appear within the original
mother cells and the number of cells in the samples increases with length of exposure to 121°C. No such increase in cells
occurs at room temperature, suggesting that the increase in daughter cells is brought about by exposure of the Red Rain
cells to high temperatures. This is an independent confirmation of results reported earlier by two of the present authors,
claiming that the cells can replicate under high pressure at temperatures upto 300°C. The flourescence behaviour of the
red cells is shown to be in remarkable correspondence with the extended red emission observed in the Red Rectagle
planetary nebula and other galactic and extragalactic dust clouds, suggesting, though not proving an extraterrestrial
origin.
A key result of hydrogravitational dynamics cosmology relevant to astrobiology is the early
formation of vast numbers of hot primordial-gas planets in million-solar-mass clumps as the dark
matter of galaxies and the hosts of first life. Photon viscous forces in the expanding universe of the
turbulent big bang prevent fragmentations of the plasma for mass scales smaller than protogalaxies.
At the plasma to gas transition 300,000 years after the big bang, the 107 decrease in kinematic
viscosity ν explains why ~3x107 planets are observed to exist per star in typical galaxies like the
Milky Way, not eight or nine. Stars form by a binary accretional cascade from Earth-mass
primordial planets to progressively larger masses that collect and recycle the stardust chemicals of
life produced when stars overeat and explode. The astonishing complexity of molecular biology
observed on Earth is possible to explain only if enormous numbers of primordial planets and their
fragments have hosted the formation and wide scattering of the seeds of life virtually from the
beginning of time. Geochemical and biological evidence suggests that life on Earth appears at the
earliest moment it can survive, in highly evolved forms with complexity requiring a time scale in
excess of the age of the galaxy. This is quite impossible within standard cold-dark-matter
cosmology where planets are relatively recent, rare and cold, completely lacking mechanisms for
intergalactic transport of life forms.
We show that radiogenic heating in primordial comets of radii in excess of ~10km could produce liquid water cores
persisting for hundreds of thousands to millions of years. Supposing comets were seeded with even the smallest
numbers of viable microbes at the time of their formation from pre-solar material, there is ample time for exponential
amplification within the liquid interiors before refreezing occurs. Freeze-dried biological material is returned to
interplanetary and interstellar space during cometary activity as the outer layers of comets are stripped away via
sublimation. Modelling of the post-impact 8-12μm spectra of Tempel 1 gives a strong indication of mixtures of clays
and organics in comparable quantity, clays in turn providing evidence of a liquid water history of the comet. The totality
of comets in a galaxy or a cluster of galaxies, seems to provide a far more promising setting for an origin of life than any
setting thus far proposed in relation to the primitive Earth. Once life has originated in a comet mechanisms of interstellar
panspermia that have recently been identified will disperse throughout the Galaxy within a few billion years.
Keywords: Comets, liquid water, clay, organics, origin of life, panspermia
We review the current state of knowledge concerning microbial extremophiles and comets and the potential significance of comets to Astrobiology. We model the thermal history of a cometary body, regarded as an assemblage of boulders, dust, ices and organics, as it approaches a perihelion distance of ~ 1AU. The transfer of incident energy from sunlight into the interior leads to the melting of near surface ices, some under stable porous crust, providing possible habitats for a wide range of microorganisms. We provide data concerning new evidence for indigenous microfossils in CI meteorites, which may be the remains of extinct cometary cores. We discuss the dominant microbial communities of polar sea-ice, Antarctic ice sheet, and cryoconite environments as possible analogs for microbial ecosystems that may grow in sub-crustal pools or in ice/water films in comets.
The presence of viable, but non-cultureable, bacteria on membranes through which strastopheric air samples were passed has been confirmed using viable fluorescent staining. The results are discussed in relation to the likely origin of the observed organisms.
Data acquired from various scientific disciplines in the past five years have converged to elevate the status of panspermia as one of the major contenders in theories of the origins of life on Earth. We review the trends that point towards a vindication of the idea that biomaterial from comets may be distributed widely throughout the universe and might even be reaching us in the present day.
Samples of air removed from the stratosphere, at an altitude of 41km, were previously found to contain viable, but non-cultureable bacteria (cocci and rods). Here, we describe experiments aimed at growing these organisms, together with any others, present in the samples. Two bacteria (Bacillus simplex and Staphylococcus pasteuri) and a single fungus, Engyodontium albus (limber)de Hoog were isolated from the samples. Contamination can never be ruled out when space-derived samples are studied on earth, however, we are confident that the organisms isolated here originated from the stratosphere.
The conceptual boundaries of life are rapidly expanding far beyond the confines of our planet to encompass an ever-widening region of the universe. Complex organic molecules in interstellar dust and comets appear most plausibly to be biologically derived, or at least closely related spectroscopically and structurally to such material. A de novo origin of life from non-living material is reckoned to have so minuscule a probability that its occurrence once in the universe can be considered miracle enough. The widespread distribution of similar material (e.g with the characteristics of the diffuse infrared bands and 2175 absorption features) throughout the galaxy and in external galaxies adds weight to the theory of panspermia, where it is supposed that the components of life at a generic level are readily transferred from one place to another. Spectroscopic evidence consistent with life extends to redshifts z=0.83, and from elemental abundance studies alone (e.g, of C, O and metals) in distant galaxies the possibility of cosmic life extends to redshifts as high as z=2.7.
Air samples collected aseptically over tropical India at various stratospheric altitudes ranging from 20 to 41 km using cryosampler assemblies carried on balloons flown from Hyderabad have shown evidence of living microbial cells. Unambiguous evidence of living cells came from examining micropore filters on which the samples were recovered with the use of voltage sensitive lipophilic dyes that could detect the presents of active cells. Clumps of viable cells were found at all altitudes using this technique, and this conclusion was found to be consistent with images obtained from electron microscopy. Since the 41 km sample was collected well above the local tropopause, a prima facie case for a space incidence of these microorganisms is established. Further work on culturing, PCR analysis and isotopic analysis is in progress.
An excess red emission of interstellar dust observed over the waveband 5000-8000A is well fitted in terms of photoluminescence of biologically derived chromophores. The redness of the class of Edgeworth-Kuiper belt objects and of areas of the Martian surface may have a similar explanation.
The concept of life being a cosmic phenomenon is rapidly gaining support, with new evidence from space science, geology and biology. On this picture life on Earth resulted from the introduction of bacteria from comets and the subsequent evolution of life required the continuing input of genes from comets. By analogy, the case for similar evolutionary developments elsewhere in the universe is explored. The prospects for SETI and Optical SETI are examined in relation to the theory of panspermia.
Air samples are to be collected at various altitude sin the stratosphere using balloons flown form Hyderabad, India. The samples will be passed through sterile micropore filters, after which the filters will be analyzed using voltage sensitive lipophilic dyes to detect the presence of either active or non-active cells. Organisms detected in this manner will be studied using static mass spectroscopy to establish isotropic ratios 13C/12C and D/H, which would distinguish between terrestrial and extraterrestrial cells.
Panspermia, an ancient theory, was revived in its modern form by two of the present authors (H-W) in a series of publications over the period 1977 to the present day. Unpopular at first, it is now slowly gaining popularity and is coming to be discussed, albeit with a measure of apprehension, as a serious scientific possibility. A brief resume will be given of the modern scientific case for panspermia, indicating that astronomical, geological and biological evidence is moving slowly in the direction of a paradigm shift.
The central regions of galaxies could provide the most promising venues for the large-scale synthesis of prebiotic molecules by Miller-Urey type processes.Exploding supermassive stars would produce the basic chemical elements necessary to form molecules in high-density mass flows under near-thermodynamic conditions. Such molecules are then acted upon by X-rays in a manner that simulates the conditions required for Miller-Urey type processing. The Miller-Urey molecular products could initially lead to the origination and dispersal of microbial life on a cosmological scale. Thereafter the continuing production of such molecules would serve as the feedstock of life.
Astronomical spectra over a wide range of wavelengths is reviewed and compared with predictions from organic models of interstellar and cometary grains. The data requires the widespread occurrence of functional groups involving H,O,C,N in the form of complex structures in the proportions that characterize biomaterial. We argue for the widespread occurrence of a microbiological system on a galaxy-wide scale.
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