Combining the concept of structured illumination with two-photon microscopy, two-photon grid scanning pattern
microscopy (TP-GPSM) was demonstrated to provide optical section power due to two-photon excitation and super-resolution
in lateral through structured illumination. Based on a laser scanning geometry, the two-photon illumination
patterns were effectively produced by temporally and spatially modulating the excitation light. Several possible ways to
produce structured patterns were proposed in this paper. For image reconstruction, sufficient phase-stepped images were
needed. With the 2-dimensional grid scanning pattern, ten images, including two orientations and five images with
different phases in each orientation are required, and a 2-fold improvement in lateral resolution can be obtained. TP-GPSM
was shown to have the potential for super-resolution imaging in thick tissues.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.