Dr. Donald J. Pleshinger Profile

Dr. Donald J. Pleshinger

at Univ of South Alabama

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Author

Publications (7)

Proceedings Article | 4 March 2022 Presentation + Paper

Measurement of agonist-induced Ca2+ signals in human airway smooth muscle cells using excitation scan-based hyperspectral imaging and image analysis approaches

Santina Johnson, D. Pleshinger, Josephine Jalkh, Zara Ijaz, Naga Annamdevula, Andrea Britain, C. Michael Francis, Deepak Deshpande, Silas Leavesley, Thomas Rich

Proceedings Volume 11964, 119640J (2022) https://doi.org/10.1117/12.2608276

KEYWORDS: Calcium, Hyperspectral imaging, Image analysis, Optical filters, Image filtering, Signal detection, Microscopy, Tunable filters, Spatial filters

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Proceedings Article | 4 March 2022 Paper

Improving visualization of cAMP gradients using algorithmic modelling

Patrick Howze, Naga Annamdevula, AnhVu Phan, D.J. Pleshinger, Thomas Rich, Silas Leavesley

Proceedings Volume 11964, 119640M (2022) https://doi.org/10.1117/12.2607772

KEYWORDS: Fluorescence resonance energy transfer, Data modeling, Visualization, Interference (communication), Finite element methods, Signal to noise ratio, Hyperspectral imaging, Lung, Signal detection, Sensors

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Proceedings Article | 4 March 2022 Presentation + Paper

Novel hyperspectral imaging approaches allow 3D measurement of cAMP signals in localized subcellular domains of human airway smooth muscle cells

Madison Howard, Naga Annamdevula, D. Pleshinger, Santina Johnson, Luke Beech, Raymond Penn, C. Michael Francis, Thomas Rich, Silas Leavesley

Proceedings Volume 11964, 119640I (2022) https://doi.org/10.1117/12.2608267

KEYWORDS: Fluorescence resonance energy transfer, Signal detection, Visualization, Imaging spectroscopy, Amplifiers, Hyperspectral imaging, Smoothing, Microscopes, Confocal microscopy, Venus

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Proceedings Article | 2 March 2022 Presentation + Paper

Hyperspectral imaging and adaptive thresholding to identify agonist-induced cAMP signals in pulmonary microvascular endothelial cells

N. Annamdevula, S. Johnson, D. Pleshinger, S. Castleberry, W. Russel, A. Britain, C. M. Francis, T. C. Rich, S. J. Leavesley

Proceedings Volume 11966, 1196604 (2022) https://doi.org/10.1117/12.2608292

KEYWORDS: Fluorescence resonance energy transfer, Hyperspectral imaging, Algorithm development, Signal to noise ratio, Image analysis, Vehicle control, Imaging spectroscopy, Visualization, Sensors, Computer programming

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Proceedings Article | 5 March 2021 Poster + Presentation + Paper

Effects of tissue autofluorescence on FRET efficiency estimates

D. J. Pleshinger, Naga Annamdevula, Silas Leavesley, Thomas Rich

Proceedings Volume 11649, 1164919 (2021) https://doi.org/10.1117/12.2583284

KEYWORDS: Fluorescence resonance energy transfer, Tissues, Biological research, Fluorescent proteins, Visible radiation, Venus, Resonance energy transfer, Molecular energy transfer, Kidney, Image analysis

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Proceedings Article | 5 March 2021 Presentation + Paper

Assessing drug-cell and drug-tissue interactions through spectral FRET imaging

Silas Leavesley, Naga Annamdevula, Aron Geurts, D. J. Pleshinger, Thomas Rich

Proceedings Volume 11624, 116240D (2021) https://doi.org/10.1117/12.2583249

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Proceedings Article | 17 February 2020 Presentation + Paper

Comparison of spectral FRET microscopy approaches for single-cell analysis

Joshua Deal, Naga Annamdevula, Donald Pleshinger, John Robert Griswold, Aliyah Odom, Alia Tayara, Malvika Lall, Craig Browning, Marina Parker, Thomas Rich, Silas Leavesley

Proceedings Volume 11243, 112430Y (2020) https://doi.org/10.1117/12.2546308

KEYWORDS: Fluorescence resonance energy transfer, Confocal microscopy, Microscopy, Molecules, Luminescence, Molecular energy transfer, Data acquisition, Electronic filtering, Optical filters, Hyperspectral imaging

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Showing 5 of 7 publications

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