Mr. Daniel B. Masters Profile

Daniel B. Masters

at Vanderbilt Univ

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Proceedings Article | 23 February 2010 Paper

Effects of temperature on fluorescence in human tissue

D. Masters, Alex Walsh, A. Welch, Anita Mahadevan-Jansen

Proceedings Volume 7562, 75620V (2010) https://doi.org/10.1117/12.842488

KEYWORDS: Luminescence, Tissues, Optical properties, In vitro testing, In vivo imaging, Temperature metrology, Skin, Monte Carlo methods, Tissue optics, Breast

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The fluorescence properties of human tissue are known to be temperature dependent. The most apparent effect of this dependence is the inverse relationship between fluorescence and temperature. In this study, we used fluorescence and diffuse-reflectance spectroscopy to investigate the effects of temperature on fluorescence, thermal coagulation, and tissue optical properties. Human tissue from the breast and abdomen were examined in vitro, and human skin was examined in vivo using a fluorescence and diffuse-reflectance system to observe the effects of temperature on fluorescence and optical properties. Fluorescence measurements were carried out using a pulsed nitrogen laser at 337 nm for excitation and a thermal camera for temperature measurements. Thermal variation of the specimens was provided by a phosphate buffered saline solution for the in vitro experiments and an ice pack and heat lamp for the in vivo experiments. In vitro temperatures varied from 0°C to 70°C and in vivo temperatures varied from 15°C to 40°C. Optical property measurements and Monte Carlo simulations were carried out on the in vitro samples for different levels of thermal exposure. Results of both the in vivo and in vitro experiments indicate that optical properties of human tissue change at high temperatures, primarily due to increased scattering. In addition, certain internal processes may have contributed to a decrease in fluorescence with increasing temperature. Some of these effects were found to be reversible before a certain temperature threshold, while some effects of coagulation on fluorescence and optical properties were not reversible.

Proceedings Article | 12 February 2010 Paper

The effect of temperature of fluorescence: an animal study

Alex Walsh, Bart Masters, Duco Jansen, A. Welch, Anita Mahadevan-Jansen

Proceedings Volume 7562, 75620W (2010) https://doi.org/10.1117/12.842578

KEYWORDS: Luminescence, Tissues, Temperature metrology, Optical properties, Diffuse reflectance spectroscopy, Skin, Eye, Collagen, Cameras, Tissue optics

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The effect of temperature on the fluorescence of enucleated porcine eyes and rat skin was studied. The fluorescence peak intensity was found to decrease as the tissue temperature increased. A dual-excitation, fiber-based system was used to collect fluorescence and diffuse-reflectance spectra from the samples. A thermal camera was used to determine the temperature of the tissue at the time of fluorescence measurement. The samples were mounted in a saline bath and measurements were made as the tissue temperature was increased from -20°C to 70°C. Results indicate that temperature affects several fluorescence spectra characteristics. The peak height decreased as temperature increased. At temperatures above 60°C, the peak position shifted to lower wavelengths. Heating and cooling experiments of the rat skin demonstrate the recovery of the loss in fluorescence. The diffuse reflectance spectra indicated a change in optical properties past 60°C, but prior to the denaturation temperature for collagen at 57°C, no change in optical properties was observed. Results suggest that the decrease in fluorescence is both a property of fluorescence and a result of altering optical properties.

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