Biochemical analysis and clinical tests like glucose, hemoglobin, cholesterol, iron, etc. are crucial for early illness diagnosis like diabetes, anemia and coronary deceases. These tests usually are done in state of the art instruments in well equipped laboratories in health centers. In some cases, these instruments are not portable, so they are not recommended for clinical field studies in remote areas. The present work shows a portable low-cost prototype of multi-well plates reader designed for clinical analysis. A Light Emission Diodes (LEDs) array is used as excitation source and an inexpensive webcam as detector. The light source illuminates the 96 well plates and the webcam take the image with 640x480 pixels. The data is acquired and processed by using a portable computer. 96 samples can be read including blanks and calibration standards simultaneously. Light absorption data are processed using a MatLab software designed in our laboratory to obtain calibration curves, standards lectures and samples concentration. The system was evaluated using different analytes series solutions: Neutral Red, Cooper (II) Ammonia Complex and Methyl Orange. The results shows that it is possible to measure few micro liters of solutions with adequate exactitude and precision of less than 3%. As possible analytical clinical application, iron determination was performed using Fe(III) Thiocyanate complex. This method is usually applied in serum samples analysis. The sensibility achieved with the proposed instrumentation configurations allows the analysis of iron in serum samples in the references values normal range (0.75 - 1.5 mg/L) in human.
A comparative study of the second harmonic generation at the M(H2,Ni(II),V=O)-porphyrin/glass interface in a total reflection geometry is presented. The state of polarization of the incident pulses was changed by a computer controlled rotation of a quarter wave plate. The rotation patterns obtained from the experimental data were fit to a Fourier series based upon theoretical Fourier coefficients. Complex valued components of the superficial susceptibility tensor are showed. From these values, average tilt angles concentration dependent were obtained. A different behavior for each Metalloporphyrin was observed.
Resonant molecular optical second harmonic generation (SHG) has been obtained from [2, 3, 7, 8, 12, 13, 17, 18- Octaethyl-porphinato] vanadyl, (OEP)V=O, adsorbed on the hypotenuse face of a glass prism. Rotational intensity patterns are showed by plotting the second harmonic intensity as a function of the incident polarization angle of the fundamental wave, at a fixed angle of incidence. Evaluation of these patterns by Fourier analysis allowed the determination of the second order susceptibilities components of the adsorbed layers. Assuming a delta distribution function, the mean orientation angles from normal to the surface plane, were obtained for different solution concentrations. Our results indicate an abrupt change in the angle for two concentration ranges.
KEYWORDS: Image processing, Video, Process control, Digital signal processing, Motion controllers, Video processing, Cameras, Optical alignment, Computer programming, 3D image processing
A problem common to automated assembly in manufacturing or in automated docking of spacecraft is angular and lateral alignment of components. A hybrid video system utilizing both conventional imaging and 3-D video moiré has been developed to automatically align a test target with three translational and two rotational degrees of freedom. Alignment was demonstrated via computer controlled translation and rotation stages. The video moiré system is operated in an error map mode, in which a structurally illuminated reference surface is used to chromakey the image of an identical structurally illuminated alignment target. The output is a moiré image generated by the misalignment of the two surfaces; further processing indicates the degree and direction of the misalignment. The translation alignment error signals (x, z, zoom) are generated by conventional image processing operating on filtered or non-structurally illuminated images. In one design, translational and rotational alignments are handled by separate processors controlled by a single host, and might use synthesized images as reference: the off center and smaller image of a laterally misaligned and distant target can be zoomed and centered with respect to a synthetic image. In another design, the synthetic image is passed to the angular alignment program while translational alignment takes place, so that coarse angular alignment can proceed essentially in parallel with the lateral and range alignment. Thus both sequential and parallel processing can be utilized to solve the alignment problem.
Molecular absorption and fluorescence studies of Asphaltenes in toluene solution and thin films absorbed onto glass plates were carried out. The fluorescence was induced by two different type of laser: a CW Ar+ and Nd-Yag laser. A red shifted was observed in both: fluorescence and absorption spectra, which can be attributed to asphaltenes aggregation. Experiments made with sample films in glass plate with the 532 nm Nd-Yag laser, shows a very narrow band at 566.1 nm (1126 cm-1) but with higher relative intensity compare to those obtained with CW laser, which is attributed to a Raman line. This signal can be employed to perform adsorption studies onto solid surfaces.
In this work we present an application of the forward-angle light scattering (FAST) for the flocculation point determination in asphaltene-toluene solutions. This technique allows to obtain an accurate and precise flocculation point determination compare to those obtained by traditional techniques. Estimations of flock dimensions based in the FAST measurements are presented.
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