Dr. Helen M. McEvoy Profile

Dr. Helen M. McEvoy

Student at Dublin City Univ

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Proceedings Article | 3 June 2005 Paper

Novel polymer platform for enhanced biochip performance

Helen McEvoy, Robert Blue, Nigel Kent, Lubos Polerecky, Colette McDonagh, Brian MacCraith

Proceedings Volume 5826, (2005) https://doi.org/10.1117/12.606423

KEYWORDS: Luminescence, Polymers, Sensors, Biosensors, Glasses, Fluorescence spectroscopy, Interfaces, Optical biosensors, Molecules, CMOS cameras

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We report the development of enhanced optical platforms for fluorescence-based biosensors. A previous analysis by us has shown that the emission of fluorescence in such a system is highly anisotropic and is preferentially emitted into the substrate over a well-defined angular range, with the result that the light is guided along the substrate via total internal reflection. However, conventional optical biosensors based on fluorescence detection typically employ a detector that is positioned either directly above or directly below the biochip. As a consequence, only a small fraction of the total emitted fluorescence is detected, which impacts adversely on sensor performance. The enhanced biosensor presented here is based on a novel, generic platform specifically designed to overcome the inherent limitations of planar substrates. The platform incorporates custom-designed optical elements, the purpose of which is to redirect the emitted fluorescence onto a detector positioned beneath the biochip. Platforms were fabricated using the polymer processing technique of microinjection moulding. In this paper we demonstrate the ability of this optical system to achieve a 80-fold luminescence capture enhancement. We also demonstrate its effectiveness as an enhanced biosensor platform by carrying out a proof of principle BSA/antiBSA competitive assay. This work has significant implications for the development of mass-producible, highly efficient optical biosensors.

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