The near infrared (NIR) part of the infrared synchrotron beam is usually discarded to improve the signal to noise ratio of spectral imaging at the Australian Synchrotron. In this study, NIR synchrotron beam has been extracted and used for three-dimensional (3D) imaging. A Fresnel zone aperture (FZA) was fabricated on barium fluoride windows using femtosecond ablation. The 3D point spread functions (PSFs) were recorded using the FZA mounted between the pinhole and the image sensor. An object is then placed within the boundaries of the PSF library and an object intensity distribution was recorded. Computational reconstruction methods were applied to reconstruct the object information.
The near infrared (NIR) part of the infrared synchrotron beam is usually dumped to improve the signal to noise ratio of spectral imaging. In this study, this NIR synchrotron beam has been extracted and used for three-dimensional (3D) phase imaging. A pinhole was inserted in the path of the fork shaped NIR synchrotron beam and the Airy diffraction pattern was aligned with biochemical samples and the diffracted intensity distribution was captured using an image sensor sensitive to NIR. A phase retrieval algorithm was used to estimate the 3D phase distribution at the object plane from the recorded intensity distribution.
Infrared (IR) microspectroscopy is a powerful molecular fingerprinting tool widely used for the identification of structural and functional composition of biological and chemical samples. The IR microspectroscopy beamline at the Australian Synchrotron can be operated either with a single-point narrow-band mercury cadmium telluride (MCT) detector or a focal plane array (FPA) imaging detector with 64 × 64 pixels. For the implementation of indirect nonscanning imaging technology, the system was operated with the FPA detector. In this study, we propose an indirect IR imaging technique based on the principles of correlation optics using diffractive optical elements such as random pinhole array (RPA) and Fresnel zone plate (FZP). The spatial and spectral variations of point spread functions (PSFs) of the RPA and FZP were simulated for the synchrotron configuration. Intensity responses for 2D objects were simulated using the same simulation conditions and reconstructed using Lucy-Richardson algorithm. Fabrication of diffractive elements for IR wavelengths is often a challenging task as the IR transparent material substrates, such as barium fluoride and calcium fluoride, are highly susceptible to thermal shocks and brittle by nature. The diffractive elements were fabricated by ablating directly on a 100 nm thick gold coated substrate using femtosecond laser pulses. The simulation results and the fabrication outcomes demonstrate the feasibility of indirect imaging at the synchrotron IR beamline.
Malus (1809) and Beer-Lambert (1729) laws can be combined to separate birefringence and dichroism contributions in IR hyperspectral imaging. This is achieved by using two optically aligned polarisers for the highest transmittance. By rotation sample between the two aligned polarisers, spectra are taken at several angles for a better fit. This method is shown to resolve orientation of sub-diffraction patterns which are ~50 times smaller than the diffraction limit of 5:1 μm (at the wavelength 3.3 μm). Application potential for very different fields ranging from microscopy of bio- and pharmaceutical materials (silk and paracetamol) to satellite imaging of ocean waves with altimeter at K∝ band of 35.75 GHz or 8.39 cm wavelength will be discussed.
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