Age-related macular degeneration (AMD) is a leading cause of global irreversible vision loss, with wet AMD responsible for over 90% of AMD-related vision loss. The laser-induced CNV mouse model is commonly used to study wet AMD. For this study, a functional retinal imaging system was used to investigate the pathological characteristics of laser-induced CNV in mice, providing advanced resolution to visualize structural, vascular, and molecular-specific features of the lesions and outer retinal layers.
The proposed preclinical study investigates pathological characteristics of retinal diseases such as Age-related Macular Degeneration (AMD) with transgenic small animal models using a multi-modal functional small animal retinal imaging system. For characterizing the animal models, we visualize the melanin concentration, lipofuscin accumulation, and choriocapillaris using a single imaging system. The system implements Polarization-Sensitive Optical Coherence Tomography (PS-OCT), fluorescence Scanning Laser Ophthalmoscope (fSLO), and Sensorless Adaptive Optics (SAO) for the visualizations of pathological features. As preliminary data, we acquired three different mice models and visualized the outer retinal thickness and melanin concentration. The newly developing system is expected to provide multilateral perspectives for further studies in AMD, enabling vision scientists to investigate the correlations between melanin, lipofuscin, and choriocapillaris for the root cause of AMD.
For this study, we developed a multi-modal functional retinal imaging system for small animal models to investigate representative AMD mice models. The system can simultaneously acquire information on melanin concentration, lipofuscin accumulation, and choriocapillaris. The newly developed system is expected to provide multilateral perspectives for further studies in AMD, enabling vision scientists to investigate the correlations between melanin, lipofuscin, and choriocapillaris for the root cause of the disease.
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