Juan Soto, Fabio Aguilar Mora, José Rodrigo, Yana Geng, Nshunge Musheshe, Manon Buist-Homan, Frank Lezoualc'h, Xiaodong Cheng, Martina Schmidt, Han Moshage, Tatiana Alieva
Refractive index tomography compatible with conventional microscope is used for analyzing primary rat hepatocytes injury induced by pharmacological treatments. We found that mitochondria malfunctioning is correlated with refractive index variation.
Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limited-angle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a nonhomogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.
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