Chemotherapy is one of the treatment methods aimed at killing cancer cells and hindering their metastasis. However, chemotherapy is known to induce multiple adverse side-effects. One of them is peripheral neuropathy, which causes significant discomfort to the cancer patient. It is hypothesized that neuropathy is directly linked to peripheral nerve damage or elasticity change. In this study we have attempted to use Brillouin spectroscopy to evaluate nerve elasticity changes and correlate them to the effects of chemotherapy.
SignificancePeripheral nerves are viscoelastic tissues with unique elastic characteristics. Imaging of peripheral nerve elasticity is important in medicine, particularly in the context of nerve injury and repair. Elasticity imaging techniques provide information about the mechanical properties of peripheral nerves, which can be useful in identifying areas of nerve damage or compression, as well as assessing the success of nerve repair procedures.AimWe aim to assess the feasibility of Brillouin microspectroscopy for peripheral nerve imaging of elasticity, with the ultimate goal of developing a new diagnostic tool for peripheral nerve injury in vivo.ApproachViscoelastic properties of the peripheral nerve were evaluated with Brillouin imaging spectroscopy.ResultsAn external stress exerted on the fixed nerve resulted in a Brillouin shift. Quantification of the shift enabled correlation of the Brillouin parameters with nerve elastic properties.ConclusionsBrillouin microscopy provides sufficient sensitivity to assess viscoelastic properties of peripheral nerves.
Poor recovery of peripheral neuron damage caused by trauma is currently among the most common causes for low quality of life among US population. Disabilities caused by neuron damage and poor recovery tend to cause early workforce retirement, creating a gap in skilled workforce in the US. Recent studies suggest that biomechanical vectors play an important role in nerve recovery and functionality restoration. This preliminary study focuses on investigating of correlation between biomechanical properties of murine sciatic nerve and Brillouin elasticity data. Combined Brillouin-Raman spectroscopy was used as a label-free method of elasticity measurement.
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