Dr. Lei G. Wang Profile

Dr. Lei G. Wang

at Oregon Health & Science Univ

SPIE Involvement:

Editorial Board Member: Biophotonics Discovery | Author

Publications (33)

Proceedings Article | 13 March 2024 Presentation

In situ therapeutic response imaging using a novel fluorescence imaging platform: TRIPODD

Nathan McMahon, Allison Solanki, Lei Wang, Antonio Montaño, Summer Gibbs

Proceedings Volume PC12846, PC1284604 (2024) https://doi.org/10.1117/12.3000414

KEYWORDS: Tumors, Image segmentation, Tissues, Cell death, Proteins, Fluorescence imaging, Cancer, Resistance, Tumor growth modeling, Tolerancing

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Proceedings Article | 13 March 2024 Presentation

Can near infrared nerve-specific imaging assess nerve integrity?

Anas Masillati, Antonio Montaño, Connor Barth, Lei Wang, Summer Gibbs

Proceedings Volume PC12825, PC128250I (2024) https://doi.org/10.1117/12.3004839

KEYWORDS: Nerve, Injuries, Fluorophores, Surgery, Visualization, Near infrared, Fluorescence intensity, Electromyography, Nerve regeneration, Medicine

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Proceedings Article | 13 March 2024 Presentation

Development of near infrared contrast agents for non-invasive early-stage cancer detection

Grace Hubbell, Riley Whalen, Heather Theison, Joshua Pace, Junxiong Ma, Melissa Wong, Mark Niedre, Lei Wang, Summer Gibbs

Proceedings Volume PC12862, PC1286204 (2024) https://doi.org/10.1117/12.3003333

KEYWORDS: Cancer detection, Contrast agents, Near infrared, Cancer, Blood, Tumors, Oncology, Liquids, Flow cytometry, Diagnostics

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Proceedings Article | 13 March 2024 Presentation

Paired-agent imaging for measuring drug-target interactions in vivo

Kimberley Samkoe, Jonathan Elliott, Lei Wang, Summer Gibbs, Kenneth Tichauer

Proceedings Volume PC12821, PC1282102 (2024) https://doi.org/10.1117/12.3008552

KEYWORDS: In vivo imaging, Proteins, Therapeutics, Oncology, Molecules, Cell death, Animal model studies, Therapeutic agents, Solubility, Perfusion imaging

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Proceedings Article | 13 March 2024 Presentation

IDH targeted fluorescence guided surgery in low grade gliomas

Linton Evans, Lei Wang, Augustino Scorzo, Rendall Strawbridge, Scott Davis, Summer Gibbs

Proceedings Volume PC12825, PC128250S (2024) https://doi.org/10.1117/12.3023422

KEYWORDS: Fluorescence, Surgery, Tumors, Target designation, Resection, Molecules, Blood brain barrier, Toxicity, Proteins, In vitro testing

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Proceedings Article | 13 March 2024 Presentation

Quantitative fluorescence imaging of drug distribution and therapeutic response at single-cell resolution

Lei Wang, Antonio Montano, Nathan McMahon, Allison Solanki, Kimberley Samkoe, Kenneth Tichauer, Summer Gibbs

Proceedings Volume PC12821, PC128210D (2024) https://doi.org/10.1117/12.3003598

KEYWORDS: Tissues, Fluorophores, Fluorescence imaging, Therapeutics, Molecules, Biological imaging, Visualization, Live cell imaging, Fluorescence, Dyes

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Proceedings Article | 13 March 2024 Presentation

Prospects for fluorescence molecular in vivo liquid biopsy of circulating tumor cells

Joshua Pace, Madduri Srinivasarao, Shivakrishna Kallepu, Riley Whalen, Heather Theison, Grace Hubbell, Lei Wang, Melissa Wong, Summer Gibbs, Philip Low, Mark Niedre

Proceedings Volume PC12825, PC128250A (2024) https://doi.org/10.1117/12.3002790

KEYWORDS: In vivo imaging, Fluorescence, Cancer detection, Tumors, Liquids, Contrast agents, Biopsy, Blood, Near infrared, Cancer

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Proceedings Article | 13 March 2024 Presentation

Development of near-infrared fluorophores for improved nerve-sparing surgical procedures

Lei Wang, Connor Barth, Antonio Montaño, Anas Masillati, Dani Szafran, Summer Gibbs

Proceedings Volume PC12862, PC1286205 (2024) https://doi.org/10.1117/12.3003618

KEYWORDS: Nerve, Fluorophores, Near infrared, Visualization, Surgery, Nervous system, Injuries, Imaging systems, Contrast agents, Real time imaging

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Proceedings Article | 17 March 2023 Presentation

Quantitative fluorescence imaging of tyrosine kinase inhibitors in live cells and tissues (Conference Presentation)

Lei Wang, Antonio Montaño, Allison Solanki, Nathan McMahon, Kimberley Samkoe, Kenneth Tichauer, Summer Gibbs

Proceedings Volume PC12398, PC123980I (2023) https://doi.org/10.1117/12.2651093

KEYWORDS: Tissues, Luminescence, Live cell imaging, Molecules, Visualization, Therapeutics, Therapeutic agents, Rhodamine, Resistance, Receptors

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Proceedings Article | 17 March 2023 Presentation

Clinical translation of near infrared nerve-specific fluorescence contrast agents for enhanced nerve identification during surgery

Connor Barth, Lei Wang, Antonio Montaño, Anas Masillati, Syed Rizvi, Adam Alani, Alexander Antaris, Jonathan Sorger, Summer Gibbs

Proceedings Volume PC12361, PC123610J (2023) https://doi.org/10.1117/12.2651327

KEYWORDS: Nerve, Surgery, Near infrared, Luminescence, Visualization, Tissues, Lead, Toxicology, Systems modeling, Safety

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Proceedings Article | 17 March 2023 Presentation

Utilization of near infrared cranial nerve-specific fluorophores intraoperatively for visualization enhancement

Anas Masillati, Antonio Montaño, Lei Wang, Connor Barth, Dani Szafran-Reeder, Summer Gibbs

Proceedings Volume PC12361, PC123610L (2023) https://doi.org/10.1117/12.2650869

KEYWORDS: Visualization, Near infrared, Nerve, Lead, Tumors, Injuries, Tissues, Surgery, Skull, Sensors

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Proceedings Article | 17 March 2023 Presentation

Two-color imaging of vital nerve structures with applications in fluorescence-guided surgery using near-infrared fluorophores with improved physicochemical properties

Antonio Montaño, Anas Masillati, Lei Wang, Dani Szafran-Reeder, Connor Barth, Nourhan Shams, Summer Gibbs

Proceedings Volume PC12361, PC123610K (2023) https://doi.org/10.1117/12.2648521

KEYWORDS: Nerve, Surgery, Prostate cancer, Cancer, Tumors, Target designation, Proteins, Prostate, Near infrared, Lead

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Proceedings Article | 15 March 2023 Presentation + Paper

First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues

Kendra Hebert, Logan Bateman, Dylan Parker, Jenna Nunziata, Joseph Paydarfar, Jennifer Hong, Darcy Kerr, George Zanazzi, Connor Barth, Lei Wang, Summer Gibbs, Eric Henderson

Proceedings Volume 12361, 123610D (2023) https://doi.org/10.1117/12.2650748

KEYWORDS: Fluorophores, Nervous system, Nerve, Adipose tissue, Lead, Biological samples, Surgery, Injuries, Muscles, Visualization

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Proceedings Article | 15 March 2023 Presentation + Paper

Use of freshly amputated human limbs for pre-clinical evaluation of molecular-targeted fluorescent probes

Logan Bateman, Kendra Hebert, Samuel Streeter, Jenna Nunziata, Connor Barth, Lei Wang, Summer Gibbs, Eric Henderson

Proceedings Volume 12361, 1236109 (2023) https://doi.org/10.1117/12.2650356

KEYWORDS: Fluorophores, Animal model studies, Tissues, Nervous system, Nerve, Animals, Lead, Surgery, Performance modeling, Muscles

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SPIE Journal Paper | 5 January 2023 Open Access

Preclinical evaluation of molecularly targeted fluorescent probes in perfused amputated human limbs

Logan Bateman, Kendra Hebert, Jenna Nunziata, Samuel Streeter, Connor Barth, Lei Wang, Summer Gibbs, Eric Henderson

JBO, Vol. 28, Issue 08, 082802, (January 2023) https://doi.org/10.1117/12.10.1117/1.JBO.28.8.082802

KEYWORDS: Fluorophores, Nerve, Nervous system, Animal model studies, Lead, Adipose tissue, Tissues, Imaging systems, Muscles, Fluorescence imaging

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Proceedings Article | 7 March 2022 Presentation

Utilization of near infrared nerve-specific fluorescent contrast agents as an intraoperative assessment methodology for nerve damage

Anas Masillati, Summer Gibbs, Connor Barth, Lei Wang, Nourhan Shams, Antonio Montano

Proceedings Volume PC11943, PC119430C (2022) https://doi.org/10.1117/12.2626009

KEYWORDS: Nerve, Near infrared, Injuries, Tissues, Surgery, Visualization, Molecules, Lead

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Proceedings Article | 4 March 2022 Presentation + Paper

An investigation into the in vivo performance of Changsha and rhodamine fluorophores

Antonio Montaño, Lei Wang, Anas Masillati, Nourhan Shams, Summer Gibbs

Proceedings Volume 11943, 119430A (2022) https://doi.org/10.1117/12.2626051

KEYWORDS: Nerve, Rhodamine, Rhodamine B, Near infrared, Absorbance, Injuries, In vivo imaging, Surgery

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Proceedings Article | 4 March 2022 Presentation + Paper

Fine-tuning physicochemical properties of Oxazine-4 for nerve-specific imaging

William Greer, Antonio Montaño, Nourhan Shams, Dani Szafran-Reeder, Anas Masillati, Lei Wang, Summer Gibbs

Proceedings Volume 11943, 1194308 (2022) https://doi.org/10.1117/12.2612232

KEYWORDS: Nerve, Tissues, In vivo imaging, Visualization

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Proceedings Article | 4 March 2022 Presentation

Quantitative imaging of tyrosine kinase inhibitor distribution in live cells and tissues

Lei Wang, Antonio Montaño, Allison Solanki, Nathan McMahon, Kenneth Tichauer, Kimberley Samkoe, Summer Gibbs

Proceedings Volume PC11979, PC119790I (2022) https://doi.org/10.1117/12.2612233

KEYWORDS: Tissues, Live cell imaging, Molecules, Luminescence, Cancer, Visualization, Resistance, Therapeutics, Therapeutic agents, Rhodamine

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Proceedings Article | 4 March 2022 Presentation + Paper

Nonlinear unmixing to account for blood absorption in multispectral imaging for improved quantification of intracellular and extracellular EGFR

Veronica Torres, Lei Wang, Sassan Hodge, Allison Solanki, Nathan McMahon, Summer Gibbs, Kenneth Tichauer, Kimberley Samkoe

Proceedings Volume 11938, 1193805 (2022) https://doi.org/10.1117/12.2609773

KEYWORDS: Tumors, Blood, Content addressable memory, Absorption, Multispectral imaging, Luminescence, Tumor growth modeling, Receptors, Proteins, Therapeutics

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Proceedings Article | 8 March 2021 Presentation

Development of near-infrared fluorophores for use as nerve-specific contrast agents for fluorescence guided surgery

Lei Wang, Connor Barth, Antonio Montaño, Alexander Antaris, Jonathan Sorger, Summer Gibbs

Proceedings Volume 11625, 116250S (2021) https://doi.org/10.1117/12.2583286

KEYWORDS: Nerve, Luminescence, Tissues, Near infrared, Imaging systems, Visualization, Safety, Real time imaging, Molecules, Surgery

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Proceedings Article | 7 March 2021 Presentation

Water-soluble and cell-permeant zwitterionic rhodamine dyes enable quantitative imaging of drug target engagement

Antonio Montaño, Lei Wang, Allison Solanki, Nathan McMahon, Marcus Kwon, Kenneth Tichauer, Kimberley Samkoe, Summer Gibbs

Proceedings Volume 11624, 116240H (2021) https://doi.org/10.1117/12.2582638

KEYWORDS: Rhodamine, Cancer, Resistance, Molecules, Imaging systems, Visualization, Silicon, Receptors, Oncology, Dynamical systems

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Proceedings Article | 5 March 2021 Presentation

TRIPODD: a novel fluorescence imaging platform for in situ measurement of drug distribution and proteomic therapeutic response

Nathan McMahon, Allison Solanki, Lei Wang, Jocelyn Jones, Kimberly Samkoe, Kenneth Tichauer, Summer Gibbs

Proceedings Volume 11624, 116240L (2021) https://doi.org/10.1117/12.2582773

KEYWORDS: Proteins, Luminescence, In situ metrology, Tumors, Tissues, Therapeutics, Spatial resolution, Resistance, Receptors, Medicine

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Proceedings Article | 5 March 2021 Presentation

Pharmacology of near infrared nerve-specific fluorophores for fluorescence-guided nerve sparing surgical procedures

Connor Barth, Lei Wang, Antonio Montaño, Alexander Antaris, Jonathan Sorger, Summer Gibbs

Proceedings Volume 11625, 116250R (2021) https://doi.org/10.1117/12.2583265

KEYWORDS: Nerve, Near infrared, Pharmacology, Visualization, Toxicity, Safety, Animal model studies, Surgery, Luminescence, Imaging systems

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Proceedings Article | 20 April 2020 Presentation

Quantification of tissue distribution and therapeutic response using paired agent imaging in a chicken chorioallantoic membrane xenograft assay (Conference Presentation)

Kimberley Samkoe, Lei Wang, Jesse Korber, Summer Gibbs, Kenneth Tichauer

Proceedings Volume 11219, 112190E (2020) https://doi.org/10.1117/12.2547100

KEYWORDS: Tumors, Tissues, 3D modeling, Content addressable memory, In vivo imaging, Molecules, Statistical modeling

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Proceedings Article | 19 February 2020 Paper

In vivo nerve-specificity of rhodamines and Si-rhodamines

Antonio Montaño, Lei Wang, Connor Barth, Nourhan Shams, K. A. Sashini Kumarapeli, Summer Gibbs

Proceedings Volume 11222, 112220I (2020) https://doi.org/10.1117/12.2545311

KEYWORDS: Rhodamine, Nerve, Near infrared, In vivo imaging, Surgery

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Proceedings Article | 19 February 2020 Paper

Noninvasive in vivo mapping of intracellular signaling proteins using a pairing of targeted and untargeted fluorescently labeled small molecule kinase inhibitors

Kenneth Tichauer, Lei Wang, Allison Solanki, Jesse Korber, Summer Gibbs, Kimberley Samkoe

Proceedings Volume 11219, 112190K (2020) https://doi.org/10.1117/12.2545926

KEYWORDS: Information technology, Tissues, Molecules, In vivo imaging, Mathematical modeling, Imaging systems, Proteins, Cancer, Plasma, Differential equations

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Proceedings Article | 19 February 2020 Presentation + Paper

Intracellular paired agent imaging enables improved evaluation of tyrosine kinase inhibitor target engagement

Allison Solanki, Lei Wang, Jesse Korber, Nathan McMahon, Kenneth Tichauer, Kimberley Samkoe, Summer Gibbs

Proceedings Volume 11219, 112190F (2020) https://doi.org/10.1117/12.2546234

KEYWORDS: Tumors, Tissues, Proteins, Imaging systems, In vivo imaging, Cancer, Visualization, Receptors

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Targeting the aberrant epidermal growth factor receptor (EGFR) signaling pathway is an attractive choice for many cancers (e.g., non-small cell lung carcinoma (NSCLC) and head and neck squamous cell carcinoma (HNSCC)). Despite the development of promising therapeutics, incomplete target engagement and acquired resistance (e.g., mutagenesis and intracellular signaling pathway rewiring) ensure that curative options still elude patients. To address limitations posed by standard drug evaluation assays (e.g., western blot, bulk plasma monitoring, immunohistochemistry), we have developed a novel dynamic, fluorescence-based platform termed intracellular paired agent imaging (iPAI). iPAI quantifies intracellular protein target engagement using two matched small-molecule, cell membrane-permeable agents: one targeted to the protein of interest and one untargeted, which accounts for non-specific therapeutic uptake. Currently, our iPAI panel includes successfully characterized tyrosine kinase inhibitors targeting the kinase binding domain of numerous proteins in the EGFR pathway, including erlotinib (EGFR). Here, we present a pharmacokinetic uptake study using our novel iPAI erlotinib reagents: a targeted erlotinib probed conjugated to silicon tetramethylrhodamine (Erl- SiTMR-T) and an untargeted reagent conjugated to tetramethylrhodaime (Erl-TMR-UT). An initial uptake study in a cell derived xenograft (CDX) model of NSCLC was performed by administering the Erl iPAI reagents systemically via tail vein injection, where drug uptake was quantified in the tumor over time. Excitingly, evidence of heterogeneous uptake was observed in the iPAI injected cohort, displaying distinct drug-uptake within a single tumor. Characterization of additional iPAI agents targeting downstream effectors (e.g., AKT, PI3K, MEK and ERK) is ongoing and will allow us to visualize complex drug-target interactions and quantify their downstream signaling partners during treatment regimens for NSCLC and other cancers. Together, we anticipate these iPAI probes will improve understanding of current limitations in personalized cancer therapy.

Proceedings Article | 7 March 2019 Paper

Investigation of oxazine and rhodamine derivatives as peripheral nerve tissue targeting contrast agent for in vivo fluorescence imaging

Lei Wang, Connor Barth, Jason Combs, Antonio Montaño, Summer Gibbs

Proceedings Volume 10862, 108620H (2019) https://doi.org/10.1117/12.2507296

KEYWORDS: Rhodamine, Nerve, Tissues, In vivo imaging, Absorbance, Near infrared, Image-guided intervention

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Proceedings Article | 28 February 2019 Paper

Simultaneous extracellular and intracellular quantification of EGFR using paired-agent imaging in an in ovo tumor model

Kimberley Samkoe, Emily Schultz, Allison Solanki, Lei Wang, Jesse Korber, Kenneth Tichauer, Summer Gibbs

Proceedings Volume 10859, 108590F (2019) https://doi.org/10.1117/12.2510778

KEYWORDS: Tumors, Content addressable memory, Luminescence, Proteins, Molecules, Tissues, Multispectral imaging, Receptors, Control systems, Tumor growth modeling

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Proceedings Article | 14 March 2018 Presentation

Paired agent imaging enables rapid assessment of therapeutic efficacy for personalized therapy (Conference Presentation)

Emily Schultz, Allison Solanki, Lei Wang, Kenneth Tichauer, Summer Gibbs

Proceedings Volume 10475, 104750K (2018) https://doi.org/10.1117/12.2290626

KEYWORDS: Resistance, Cancer, Medicine, Pancreatic cancer, Surgery, Visualization, Oncology, Biopsy

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Proceedings Article | 14 March 2018 Presentation

Topical application of nerve-specific fluorophores for image-guided nerve sparing surgical procedures (Conference Presentation)

Connor Barth, Summer Gibbs, Lei Wang

Proceedings Volume 10478, 104780J (2018) https://doi.org/10.1117/12.2290681

KEYWORDS: Nerve, Luminescence, Image-guided intervention, Surgery, Visualization, Tissues, Near infrared, Oncology, Target designation, Molecular imaging

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Nerve damage plagues surgical outcomes, significantly affecting post-surgical quality of life. Surprisingly, no method exists to enhance direct nerve visualization in the operating room, and nerve detection is completed through a combination of palpation and visualization when possible. Fluorescence image-guided surgery offers a potential means of enhanced nerve identification and preservation, however a clinically approved nerve-specific contrast agent does not yet exist. Several classes of nerve-specific fluorophores have recently been demonstrated including the distyrylbenzenes (DSB), select oxazines (oxazine 4 perchlorate), and certain cyanines (3,3’-diethylthiatricarbocyanine iodine), which could provide intraoperative guidance. The nerve-sparing radical prostatectomy is a surgical procedure that could benefit from fluorescence image-guided nerve identification. Although the nerve-sparing surgical technique was developed over 30 years ago, nerve damage following radical prostatectomy is reported in some form in up to 60% of patients one to two years post-surgery. To facilitate clinical translation of fluorescence image guided surgery to the nerve sparing prostatectomy, a direct administration methodology was developed that allows selective nerve highlighting with a significantly lower fluorophore dose than systemic administration, where large animal studies have confirmed the technique’s translatability. Tissue penetration will be critical for clinical feasibility of the direct administration methodology and novel formulation strategies have been explored to enhance tissue penetration for identifying buried nerves. In addition, several biomolecular targets of Oxazine 4, a promising candidate for nerve-specific fluorophore development into a near-infrared (NIR) agent, have been identified, providing insight into the mechanism of nerve-specificity. Fluorophore development has made progress towards the goal of creating a NIR nerve-specific fluorophore and determining the structure-activity relationship responsible for nerve binding.

Proceedings Article | 14 March 2018 Presentation

Quantitative imaging of gemcitabine engagement and efficacy in pancreatic cancer (Conference Presentation)

Lei Wang, Allison Solanki, Kenneth Tichauer, Summer Gibbs

Proceedings Volume 10475, 104750L (2018) https://doi.org/10.1117/12.2291265

KEYWORDS: Therapeutics, Pancreatic cancer, Luminescence, Cancer, Liquids, Chromatography, Mass spectrometry

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Showing 5 of 33 publications

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