Multispectral endoscopic imaging is a promising technique for lesion detection, and surgical guidance. Based on different spectral properties of tissues, multispectral imaging can provide enhanced contrast of vascular structures (narrow band imaging) or enable the quantitative analysis of hemoglobin and lipid. The multiparameter phantoms serve as efficient tools for system calibration, performance evaluation, and algorithm development in multispectral endoscopy are needed. In this paper, we developed a multiparameter tissue-mimicking phantom that mimics the parameters of human gastric mucosa, such as scattering coefficient, scattering layer thickness, vascular width, lipid on the surface and blood oxygen saturation (SO2). We verified the SO2 measurement accuracy by comparing with commercial i-STAT devices for SO2 distribution imaging. At the same time, the segmentation of lipid regions was also tested. Our results demonstrate that this multiparameter phantom is a versatile tool that can facilitate validation and evaluation of multispectral endoscopic systems.
Polarimetric imaging can provide morphological and structural information of tissue and has demonstrated applications during endoscopy. Polarimetric endoscopy has been shown by conducting polarimetric imaging through customized rigid endoscopes. Here we demonstrate Stokes polarimetric imaging via a commercially available rigid endoscope that can acquire full-Stokes information in a snapshot. This system incorporates two division of focal plane linear polarization cameras, a waveplate and a commercially available rigid endoscope. In addition, the sapphire windows in rigid endoscopes are known for their polarization effects. In this work, the polarization properties of the sapphire windows are carefully measured and calibrated to guarantee the accuracy of the system. Quantified analysis of the system and the imaging results on tissue are provided.
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