We describe the concept of laser rastering flow cytometry, where a rapidly scanning laser beam allows counting and
classification of cells at much higher rates than currently possible. Modifications to existing flow cytometers to
implement the concept include an acousto-optic deflector, fast analog-to-digital conversion, and a two-step
digital-signal-processing scheme that handles the high data rates and provides key assay information. Results are shown that
prove the concept, demonstrating the ability to resolve closely spaced cells and to measure cells at rates more than an
order of magnitude faster than on conventional flow-cytometer-based hematology analyzers.
We simulate pulse compression mechanism based on a near-two- photon-resonance transition contribution to the nonlinear refractive index of atomic Noble gas filled hollow wave- guides. The negative refractive index contribution in the normal dispersive gas wave-guide, plays a similar role as in the case of soliton compression with positive Kerr non- linearity and anomalous dispersion in optical fibers. The self pulse compression to approximately 15 fsec can be achieved at moderate peak powers (approximately MW) for 100 fsec pulses in the spectral range 100 - 245 nm. We present simulated data concerning pulse and spectral shapes for xenon as a case study. The total throughput of the propagated pulse energy is > 90%, mostly determined by the linear attenuation of the hollow wave-guide propagation mode while two photon absorption and the corresponding enhanced three photon photo-ionization does not significantly reduce the pulse energy.
We simulate pulse compression mechanism based on a near-two- photon-resonance (NTPR) transition contribution to the nonlinear refractive index of atomic Noble gas filled hollow wave-guides. The negative refractive index contribution in the normal dispersive gas waveguide, plays a similar role as in the case of soliton compression with positive Kerr non- linearity and anomalous dispersion in optical fibers. The self pulse compression to approximately 15 fsec can be achieved at moderate peak powers for 100 fsec pulses in the spectral range 100-245 nm. We present simulated data concerning pulse and spectral shapes for xenon as a case study. The total throughput of the propagated pulse energy is > 90 percent, mostly determined by the linear attenuation of the hollow wave-guide propagation mode while two photon absorption and the corresponding enhanced three photon photo-ionization does not significantly reduce the pulse energy.
We simulate a pulse compression process in hollow wave- guides filled with atomic xenon based on the near blue side two photon resonance contribution to the nonlinear refractive index. The negative nonlinear refractive index contribution in the normal dispersion xenon gas wave-guide play s a similar role as in the case of soliton compression in optical fibers with positive nonlinear refractive index and anomalous dispersion.
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