Identification and validation of approaches for evaluation of the bioavailability of a topically applied drug in the skin represent a work-in-progress. Coherent Raman imaging can quantitatively visualize drug and elucidate pharmacokinetics estimates. Here, we imaged and quantified the uptake of tazarotene through distinct skin layers of perfused in vivo and non-perfused ex vivo nude mouse ear. Differences in the pharmacokinetic values could be seen at all skin depths and, also, between perfused non-perfused skin ears. This technique facilitates the direct observation of drug uptake into the skin layers, shedding light on the mechanisms of drug kinetics into the skin.
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