Laser speckle patterns were used for characterization of skin topography (DermaSpec, 13N13841). A set of parameters were obtained for skin surface characterization. Preliminary data has already shown differences between skin tumor and healthy skin areas.
We present the digital inline holographic microscopy for blood cell counting. The microscope unit contains a pinhole chip for illumination, a micro-fluidic chip, and a CMOS-chip for detection. The design is adapted and tested for blood cell diagnostics. A single hologram delivers a stack of images for all z-positions in the micro-fluidic channel and is the basic for counting of flowing cells. Images of flowing red blood cells are presented providing clinical diagnostics.
An in-line holography setup with pinholes is discussed. A set of coherent light cones generated by a pinhole array
illuminates the sample. Compared to a single pinhole the effective numerical aperture is increased. The axial resolution
is determined by the effective aperture and is increased, too.
Inline holography with a pinhole is extended by using a multi-spot source and a CCD. The arrangement is aimed on
microscopic imaging. By using a pinhole array as a multi-spot source the size of observable samples can be extended.
The intensity of the illumination of samples can be increased up to several orders. It enables the increase of the distance
to the CCD to get an enlarged, pixel adapted hologram pattern. Very small pinholes are applicable and the useable
aperture can be increased, too.
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