In this paper, we report the results of accuracy verification of a measurement method for capturing pulse waves from the sole of rodents using an RGB camera in a non-contact, non-invasive manner. In order to acquire biometric data such as pulse waves from animals, a non-invasive method is required to minimize the impact on the animal. We experimented with verifying the accuracy of pulse waves using an RGB camera synchronized with the electrocardiogram (ECG). As a result, our proposed method of detecting pulse waves from the sole was observed to measure pulse rate with similar accuracy to an ECG. The deviation of the heartbeat interval was somewhat larger for the proposed method than for the ECG; the maximum absorption peak of the Green signal showed the same pattern in the extremities, indicating that the deviation of pulse wave propagation was larger than 1/250 s. The deviation between the R-wave peak of the ECG and the maximum absorption peak of the sole was 0.1 s.
In this paper, we propose a measurement method using an RGB camera to non-invasively capture the pulse wave in rats. Most attempts to capture biological information from an animal, such as pulse wave, requires contact that is invasive and influence the animal. In this study, we attempt to apply remote photoplethysmography (rPPG) to rats. Our rPPG method uses an RGB camera to detect the change of hemoglobin amount in the skin and derives pulse waves. First, we removed body hair and captured an image of the skin in the hair removed area. The rat's pulse rate is approximately 300 beats per minute, which is much faster than the pulse rate of a human; then we captured video of the skin at a frame rate of 250 frames per second (fps). As a result, pulse waves were obtained from the image signals of the skin with signal processing. Next, we focused on the sole of rats whose skin is directly visible, and we tried to detect the pulse wave from the sole. We made a novel rat observation apparatus, where the cage floor is made of a transparent acrylic plate, and images of rate roles were captured through the acrylic plate by setting the RGB camera under the cage. As a result, we were able to measure pulse waves in rats by a non-contact, non-invasive, unrestrained, and no anesthesia approach. We also demonstrated the effectiveness of the proposed method we could successfully detecting arrhythmia caused by fear (fox smell).
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