The spectral-luminescent characteristics of cyanine dye Cyan 40 and thiazole orange at interaction with
biomacromolecules bovine serum albumin and deoxyribonucleic acid was studied. It is shown that presence
of biomacromolecules in solution of the studied species leads to the change of spectral-luminescent
characteristics of the dyes Cyan 40 and TO. The observed phenomena in absorption spectrum and
fluorescence of the studied dyes is explained by complex formation between dye and biological
macromolecules. The binding parameters: a binding constant (K) and quantity of the binding sites (N) the
studied dyes with biomacromolecules are determined.
It was studied the influence of F, Sbt, Sil, Sbo monomer and homodimer Dst-5, Dst-10, Dbt-5, Dbt-10, Dil-10, Dbo-10 styryl dyes on blood erythrocytes of white rats. It was shown that the homodimer styryl
dyes Dst-5, Dbt-5 and Dbo-10 decrease the erythrocytes quantity by 1.5-2 times more as compared with
monomer dyes Sbt and Sbo. The main cause of dyes different action is the different oxidation degree of
intracellular hemoglobin evoked by these dyes. It was established that the observed effects was connected
with different penetration of these dyes through membrane of erythrocytes and with interaction of these dyes
with albumin localized in membranes of cells.
The functional differences of human blood serum albumin in norm and at different patologic process were
studied by spectral-luminescent method by comparison of binding constant (K) and concentration of binding
sites (N) values of rhodamine B dye with blood serum. It was shown that K and N of rhodamine B dye with
blood serum of sick men is decreased as compared to that for healthy men.
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