A Jones matrix model of biological fluids dehydrated films (facies) optical anisotropy is proposed. The relationships between the blood facies phase (birefringence) anisotropy parameters and the Jones matrix elements real and imaginary components are determined. The experimental testing results of the blood facies polarization matrix thesiography polycrystalline architectonics method with linear and circular birefringence coordinate distributions reproduction are presented. Integral blood facies phase anisotropy maps are presented. Scenarios changes of 1st - 4th orders central statistical moments, which characterize blood facies phase anisotropy maps are determined and physically analysed. The results of the Jones matrix thesiography method clinical testing is presented. Very good and balanced accuracy level has been achieved in the temporal differentiation of the long-term outcomes of patients who have undergone COVID-19. The prospects for further research using an integrated approach based on polarization analysis of laser-induced auto fluorescent images of supramolecular networks of blood facies are indicated.
A short theory of the processes of formation of the laser speckle fields polarization structure of biological tissues with optically anisotropic architectonics is presented. A method of polarization interference mapping of speckle fields with layerby-layer phase scanning of holographically reconstructed distributions of complex amplitudes of biological preparations of various morphological structures and physiological states has been proposed and experimentally tested - biopsies of the thyroid gland and blood films of rats with different severity of sepsis. Layer-by-layer maps of polarization ellipticity of native histological sections of thyroid biopsies from donors and sick patients were studied. As part of the statistical analysis of phase coordinate distributions of polarization ellipticity, objective markers for the differential diagnosis of thyroid pathology were found - nodular goiter, autoimmune thyroiditis and papillary cancer. Very good (~90%) and excellent (≥90%) accuracy of differential diagnosis was achieved. Statistical markers for temporal monitoring of septic changes in polarization azimuth maps of polycrystalline blood films of rats were found. An example of further research using laser-induced autofluorescence polarimetry methods is presented.
A Mueller matrix model of biological tissues optical anisotropy is proposed. The relationships between the histological sections phase (birefringence) anisotropy parameters and polarization maps are determined. The experimental results of the brain and liver native histological sections polarization mapping of those who died with different durations of blunt mechanical trauma are presented. As part of the statistical analysis of the set of native samples digital microscopic images ellipticity coordinate distributions. The main time scenarios for changes in the biological tissues optically anisotropic architectonics polarization map’s topographic structure were determined. On this basis, a polarization method has been proposed for determining the duration of blunt mechanical trauma. The possibility of detecting, using statistical polarization markers, the duration of a mechanical injury for 18 hours with an accuracy of 45 to 60 minutes has been demonstrated. Prospects for further research using laser-induced autofluorescence polarimetry methods that are more sensitive at the molecular level are presented.
The methods of layer-by-layer interference phase scanning with digital holographic reproduction of azimuth and ellipticity polarization maps for differential diagnosis of benign (nodular goiter - NG) and malignant (papillary thyroid - PT) thyroid tumours are presented. All studies in representative groups of histological sections M and AC were conducted using a circular laser beam. This ensured the azimuthal invariance and repeatability of measurements of polarization maps NG and PT. Integrated, and layer-by-layer polarization maps of NG and PT were received. Values of statistical moments of 1st - 4th order, which characterize NG and PT samples coordinate distributions of azimuth and ellipticity polarization have been determined and systematized. A set of statistical parameters - diagnostic markers most sensitive to changes in the polycrystalline structure NG and PT has been determined. The results of information analysis - determination of accuracy of vector-parametric and polarization-interference methods of differentiation of benign and malignant thyroid tumours are presented.
The theoretical principles of the Mueller matrix reconstruction of the parameters of optical anisotropy (linear and circular birefringence) of the myocardium polycrystalline myosin fibrillar networks architectonics are considered. The main signs of polarization manifestations of pathological transformations of the spatially structured myosin fibrils networks have been determined. The found tomography algorithms were used to process the results of myocardium native histological sections Mueller matrix mapping of those who died with long-term consequences of COVID 19. As part of the statistical analysis of the obtained native histological sections myosin fibrillar networks maps of linear and circular birefringence the most sensitive markers (asymmetry and kurtosis) to pathological changes in the myocardium. On this basis, differential diagnosis with excellent (>95%) balanced accuracy of long-term consequences of COVID 19 has been implemented. Further promising areas of research using the principles of laser-induced autofluorescence polarimetry of biological tissues are indicated.
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