Prof. Paolo Antonio Netti Profile

Prof. Paolo Antonio Netti

Center Coordinator at Univ degli Studi di Napoli Federico II

SPIE Involvement:

Author

Publications (28)

Proceedings Article | 11 August 2023 Presentation + Paper

Presentation + Paper

Out of distribution detection in deep learning-based scattering pattern classification

David Dannhauser, Paolo Netti, Filippo Causa

Proceedings Volume 12622, 126220F (2023) https://doi.org/10.1117/12.2670208

KEYWORDS: Light scattering, Image classification, Scattering, Neural networks, Education and training, Microfluidics, Laser scattering, Deep learning, Convolutional neural networks, Cancer detection

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Proceedings Article | 27 July 2019 Presentation

Presentation

Engineering light-responsive substrates for the dynamic display of patterns of adhesive signals to control cell functions in vitro (Conference Presentation)

Paolo Netti

Proceedings Volume 11060, 110600J (2019) https://doi.org/10.1117/12.2528832

KEYWORDS: In vitro testing, Adhesives, Cytoskeletons

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Proceedings Article | 21 June 2019 Paper

Paper

Advanced label-free cellular identification in flow by collaborative coherent imaging techniques

David Dannhauser, Domenico Rossi, Maria Isabella Maremonti, Pasquale Memmolo, Pietro Ferraro, Paolo Antonio Netti, Filippo Causa

Proceedings Volume 11060, 110600X (2019) https://doi.org/10.1117/12.2530150

KEYWORDS: Scattering, Digital holography, Light scattering, Capillaries, Laser scattering, Coherence imaging, Blood, Microfluidics, Scatter measurement, Cameras

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Proceedings Article | 26 June 2017 Paper

Paper

Peripheral blood mononuclear cells analysis in microfluidic flow by coherent imaging tools

David Dannhauser, Domenico Rossi, Pasquale Memmolo, Filippo Causa, Andrea Finizio, Pietro Ferraro, Paolo Netti

Proceedings Volume 10333, 1033308 (2017) https://doi.org/10.1117/12.2270363

KEYWORDS: Polymers, Beam splitters, 3D metrology, Blood, Light scattering, Microfluidics, Phase imaging, Holography, 3D image processing, Coherence imaging, Digital holography, Pathogens

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Proceedings Article | 17 July 2015 Paper

Paper

Digital holography for recovering 3D shape of red blood cells

P. Memmolo, L. Miccio, F. Merola, O. Gennari, P. Netti, Pietro Ferraro

Proceedings Volume 9540, 95400I (2015) https://doi.org/10.1117/12.2185115

KEYWORDS: Digital holography, Blood, Holography, Optical tweezers, Microscopy, Diagnostics, Biological research, Biomedical optics, Neodymium, 3D image processing

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Proceedings Article | 22 June 2015 Paper

Paper

Wavefronts matching: a novel paradigm for three-dimensional holographic particle tracking

Pasquale Memmolo, Lisa Miccio, Francesco Merola, Paolo Netti, Pietro Ferraro

Proceedings Volume 9529, 95290C (2015) https://doi.org/10.1117/12.2185710

KEYWORDS: Particles, Wavefronts, Holography, 3D acquisition, Digital holography, 3D image processing, Microfluidics, Wavefront reconstruction, Microscopy, Stereoscopy

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Proceedings Article | 22 June 2015 Paper

Paper

Investigation on cytoskeleton dynamics for non-adherent cells under point-like stimuli

Lisa Miccio, Pasquale Memmolo, Francesco Merola, Martina Mugnano, Sabato Fusco, Antonio Paciello, Pietro Ferraro, Paolo Netti

Proceedings Volume 9529, 95290P (2015) https://doi.org/10.1117/12.2185929

KEYWORDS: Cytoskeletons, Luminescence, Optical tweezers, Latex, Holography, Digital holography, Particles, Imaging systems, Microscopes, Intelligence systems

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Proceedings Article | 22 June 2015 Paper

Paper

Sparsity promoting automatic focusing in digital holography

Pasquale Memmolo, Melania Paturzo, Bahram Javidi, Paolo Antonio Netti, Pietro Ferraro

Proceedings Volume 9525, 95250J (2015) https://doi.org/10.1117/12.2185015

KEYWORDS: Digital holography, Holograms, 3D image reconstruction, Microscopes, Digital recording, Holography, Denoising, Digital imaging, Particles, Phase retrieval

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Proceedings Article | 22 June 2015 Paper

Paper

Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique

David Dannhauser, Pasquale Memmolo, Domenico Rossi, Francesco Merola, Lisa Miccio, Filippo Causa, Pietro Ferraro, Paolo Netti

Proceedings Volume 9529, 95290D (2015) https://doi.org/10.1117/12.2185113

KEYWORDS: Light scattering, Microfluidics, Scattering, Particles, Holography, Digital holography, Cameras, 3D metrology, Refractive index, Laser scattering

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Proceedings Article | 22 June 2015 Paper

Paper

Microgels for multiplex and direct fluorescence detection

Filippo Causa, Anna Aliberti, Angela Cusano, Edmondo Battista, Paolo Netti

Proceedings Volume 9529, 952919 (2015) https://doi.org/10.1117/12.2185839

KEYWORDS: Luminescence, Fluorescence spectroscopy, Signal detection, Multiplexing, Computer programming, Target detection, Image analysis, Atmospheric particles, Diagnostics, Polymers

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Proceedings Article | 22 June 2015 Paper

Paper

Red blood cell three-dimensional morphometry by quantitative phase microscopy

F. Merola, P. Memmolo, L. Miccio, O. Gennari, M. Mugnano, P. Netti, P. Ferraro

Proceedings Volume 9529, 95290K (2015) https://doi.org/10.1117/12.2186785

KEYWORDS: Blood, Wavefronts, Wave propagation, Microscopy, Shape analysis, Digital holography, 3D image processing, Diagnostics, Holography, Image analysis

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Proceedings Article | 22 June 2015 Paper

Paper

Imaging and characterization of surface relief gratings on azopolymer by digital holographic microscopy

Alejandro Calabuig, Carmela Rianna, Vito Pagliarulo, Maurizio Ventre, Silvia Cavalli, Simonetta Grilli, Paolo Netti, Pietro Ferraro

Proceedings Volume 9529, 95290L (2015) https://doi.org/10.1117/12.2189205

KEYWORDS: Digital holography, Holography, Mirrors, Microscopy, Polymers, Atomic force microscopy, Holograms, Imaging systems, Biomedical optics, Lithography

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Proceedings Article | 12 March 2015 Paper

Paper

Design and optimization of polymer nanoshuttles for nanomedicine

Daniela Guarnieri, Enza Torino, Raffaele Vecchione, Paolo Netti

Proceedings Volume 9338, 933802 (2015) https://doi.org/10.1117/12.2077092

KEYWORDS: Polymers, Nanoparticles, Blood brain barrier, Toxicity, Brain, Tissues, Product engineering, Proteins, Coating, In vitro testing

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Current advances in nanotechnology hold the promises to greatly impact on current medical practice. Since nanometric materials interact with cells, tissue and organs at a molecular level, they may be used as probes for ultrasensitive molecular sensing and diagnostic imaging or carriers for drug and gene delivery. However, along with the excitement that has driven the development of novel nanocarriers, there have been increasing concerns regarding the risks these materials may generate. As these nanostructures are intentionally engineered to target specific cells or tissues, it is imperative to ensure their safety. The optimal design of safe and functional nanocarriers for medicine requires a better understanding of the interaction between the physical-chemistry properties of the nanoparticle surface with the complex protein machinery existing at the cell membrane. In particular the effect of the particles properties (charge, shape, protein coating) on the mechanism of cellular uptake is highly relevant both to assess the real biological risks coupled with the use of nanomaterial (nanopathology and nanotoxicology) and to engineer carriers able to improve the medical practice. The nanometric size and the surface molecular decoration may activate mechanisms of cellular uptake different from those commonly used by cells: these open the possibility to activated/modulated the membrane crossing by tuning chemical-physical properties of nanometric materials. In this work, the design and production of novel degradable polymeric nanocavities via layer-by-layer and temperature induced phase separation technology will be presented along with a detailed characterization of their in vitro performances. Furthermore, possible mechanisms of cellular uptake will be discussed and critically presented. The effect of surface bioconjugation on cell membrane crossing will be exploited and elucidated. Particular attention will be devoted to surface molecular decoration able to guide the nanoparticle throughout the cytosol.

Proceedings Article | 11 March 2015 Paper

Paper

Holographic quantitative imaging of sample hidden by turbid medium or occluding objects

V. Bianco, L. Miccio, F. Merola, P. Memmolo, O. Gennari, Melania Paturzo, P. Netti, P. Ferraro

Proceedings Volume 9336, 933612 (2015) https://doi.org/10.1117/12.2086808

KEYWORDS: Holograms, Blood, Stereolithography, Microscopy, Digital holography, Speckle, Holography, Light scattering, Microfluidics, Imaging systems

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Proceedings Article | 11 March 2015 Paper

Paper

Lab on chip optical imaging of biological sample by quantitative phase microscopy

P. Memmolo, L. Miccio, F. Merola, O. Gennari, M. Mugnano, P. Netti, P. Ferraro

Proceedings Volume 9336, 933625 (2015) https://doi.org/10.1117/12.2086806

KEYWORDS: 3D image processing, Blood, Digital holography, Diagnostics, Microscopy, 3D displays, Holography, Optical tweezers, Optical imaging, Tomography

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Proceedings Article | 8 May 2014 Paper

Paper

Investigation on cytoskeleton dynamics for no-adherent cells subjected to point-like stimuli by digital holographic microscopy and holographic optical trapping

Lisa Miccio, Francesco Merola, Pasquale Memmolo, Martina Mugnano, Sabato Fusco, Paolo Netti, Pietro Ferraro

Proceedings Volume 9129, 91291V (2014) https://doi.org/10.1117/12.2052811

KEYWORDS: Digital holography, Holography, Optical tweezers, Cytoskeletons, Microscopy, Optical tracking, Particles, Biomedical optics, Wavefronts, Optical microscopy

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Proceedings Article | 8 May 2014 Paper

Paper

3D visualization and biovolume estimation of motile cells by digital holography

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, G. Coppola, P. Netti

Proceedings Volume 9129, 91291W (2014) https://doi.org/10.1117/12.2052836

KEYWORDS: Biological research, Digital holography, Microfluidics, 3D metrology, Head, In vitro testing, Holograms, Optical tweezers, Microscopy, 3D modeling

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Proceedings Article | 8 March 2014 Paper

Paper

A new 3D tracking method for cell mechanics investigation exploiting the capabilities of digital holography in microscopy

L. Miccio, P. Memmolo, F. Merola, S. Fusco, P. Netti, P. Ferraro

Proceedings Volume 8947, 89471L (2014) https://doi.org/10.1117/12.2039698

KEYWORDS: Digital holography, Microscopy, Particles, Cell mechanics, Geometrical optics, 3D metrology, Statistical analysis, Microscopes, Algorithm development, Detection and tracking algorithms

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Proceedings Article | 4 March 2014 Paper

Paper

3D manipulation and visualization of in-vitro cells by optical tweezers and digital holographic microscopy

F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, G. Coppola, P. Netti, P. Ferraro

Proceedings Volume 8947, 89471A (2014) https://doi.org/10.1117/12.2039423

KEYWORDS: Digital holography, Particles, Holograms, Holography, Optical tweezers, Visualization, Microscopes, Biological research, Reconstruction algorithms, Head

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Proceedings Article | 23 May 2013 Paper

Paper

Fabrication of 3D tissue equivalent: an in vitro platform for understanding collagen evolution in healthy and diseased models

F. Urciuolo, G. Imparato, C. Casale, S. Scamardella, P. Netti

Proceedings Volume 8792, 87920K (2013) https://doi.org/10.1117/12.2022119

KEYWORDS: Collagen, Tissues, Second-harmonic generation, 3D modeling, In vitro testing, Ultraviolet radiation, Image analysis, Tissue engineering, Statistical analysis, Signal detection

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Proceedings Article | 23 May 2013 Paper

Paper

Small angle light scattering characterization of single micrometric particles in microfluidic flows

David Dannhauser, Giovanni Romeo, Filippo Causa, Paolo Netti

Proceedings Volume 8792, 879212 (2013) https://doi.org/10.1117/12.2022154

KEYWORDS: Particles, Light scattering, Scattering, Laser scattering, Scatter measurement, Refractive index, Mie scattering, Collimation, Gradient-index optics, CCD cameras

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Proceedings Article | 23 May 2013 Paper

Paper

New method of 3D tracking of in vitro cells by digital holographic microscopy

Pasquale Memmolo, Maria Iannone, Maurizio Ventre, Paolo Netti, Andrea Finizio, Melania Paturzo, Pietro Ferraro

Proceedings Volume 8792, 879210 (2013) https://doi.org/10.1117/12.2020566

KEYWORDS: Digital holography, Holograms, Holography, Phase contrast, Microscopy, In vitro testing, Image filtering, Image segmentation, Microscopes, Optical tracking

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Proceedings Article | 23 May 2013 Paper

Paper

Investigation on 3D morphological changes of in vitro cells through digital holographic microscopy

Pasquale Memmolo, Lisa Miccio, Francesco Merola, Paolo Netti, Giuseppe Coppola, Pietro Ferraro

Proceedings Volume 8792, 87920R (2013) https://doi.org/10.1117/12.2020570

KEYWORDS: Digital holography, Head, Holography, Microscopy, In vitro testing, Anisotropic filtering, Gaussian filters, Biological research, 3D metrology, Statistical analysis

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Proceedings Article | 23 May 2013 Paper

Paper

A new 3D tracking method exploiting the capabilities of digital holography in microscopy

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, P. Netti, P. Ferraro

Proceedings Volume 8792, 87920S (2013) https://doi.org/10.1117/12.2020924

KEYWORDS: Digital holography, Particles, Microscopy, Digital image correlation, Holography, Holograms, Video, Biological research, Microscopes, 3D image processing

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A method for 3D tracking has been developed exploiting Digital Holographic Microscopy (DHM) features. In the framework of self-consistent platform for manipulation and measurement of biological specimen we use DHM for quantitative and completely label free analysis of specimen with low amplitude contrast. Tracking capability extend the potentiality of DHM allowing to monitor the motion of appropriate probes and correlate it with sample properties. Complete 3D tracking has been obtained for the probes avoiding the issue of amplitude refocusing in traditional tracking processing. Our technique belongs to the video tracking methods that, conversely from Quadrant Photo-Diode method, opens the possibility to track multiples probes. All the common used video tracking algorithms are based on the numerical analysis of amplitude images in the focus plane and the shift of the maxima in the image plane are measured after the application of an appropriate threshold. Our approach for video tracking uses different theoretical basis. A set of interferograms is recorded and the complex wavefields are managed numerically to obtain three dimensional displacements of the probes. The procedure works properly on an higher number of probes and independently from their size. This method overcomes the traditional video tracking issues as the inability to measure the axial movement and the choice of suitable threshold mask. The novel configuration allows 3D tracking of micro-particles and simultaneously can furnish Quantitative Phase-contrast maps of tracked micro-objects by interference microscopy, without changing the configuration. In this paper, we show a new concept for a compact interferometric microscope that can ensure the multifunctionality, accomplishing accurate 3D tracking and quantitative phase-contrast analysis. Experimental results are presented and discussed for in vitro cells. Through a very simple and compact optical arrangement we show how two different functionalities can be accomplished by the same optical setup, i.e. 3D tracking of micro-object and quantitative phase contrast imaging.

Proceedings Article | 23 May 2013 Paper

Paper

Cell mechanics investigation by digital holographic microscopy

L. Miccio, P. Memmolo, F. Merola, S. Fusco, V. Embrione, P. Netti, P. Ferraro

Proceedings Volume 8792, 87921F (2013) https://doi.org/10.1117/12.2020925

KEYWORDS: Digital holography, Microscopy, Phase shift keying, Geometrical optics, Cell mechanics, Holography, Particles, Biology, Microorganisms, Biomedical optics

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Proceedings Article | 13 May 2013 Paper

Paper

Sparsity-based denoising method of wrapped-phase reconstructions in digital holography

Pasquale Memmolo, Maria Iannone, Maurizio Ventre, Paolo Netti, Andrea Finizio, Melania Paturzo, Pietro Ferraro

Proceedings Volume 8788, 878806 (2013) https://doi.org/10.1117/12.2020554

KEYWORDS: Digital holography, Denoising, Holograms, Reconstruction algorithms, Optical simulations, In vitro testing, Microfluidics, Holography, Image quality, Digital filtering

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Proceedings Article | 5 May 2012 Paper

Paper

A new iterative Fourier transform algorithm for optimal design in holographic optical tweezers

P. Memmolo, L. Miccio, F. Merola, P. Ferraro, P. Netti

Proceedings Volume 8429, 84291F (2012) https://doi.org/10.1117/12.922927

KEYWORDS: Detection and tracking algorithms, Reconstruction algorithms, Computer generated holography, Optical tweezers, Optical design, Fourier transforms, Holography, Particles, Diffraction, Microscopes

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Proceedings Article | 18 October 2004 Paper

Paper

Optical tweezers as a tool for microrheology of simplex and complex fluids

Giuseppe Pesce, Antonio Sasso, Sabatino Fusco, Assunta Borzacchiello, Paolo Netti

Proceedings Volume 5514, (2004) https://doi.org/10.1117/12.557862

KEYWORDS: Optical tweezers, Particles, Calibration, Objectives, Charge-coupled devices, Photodiodes, Microfluidics, Light scattering, Magnetism, Microscopes

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Showing 5 of 28 publications

Conference Committee Involvement (3)

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV

24 June 2019 | Munich, Germany

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials

26 June 2017 | Munich, Germany

Optical Methods for Inspection, Characterization, and Imaging of Biomaterials II

22 June 2015 | Munich, Germany

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