Sculpted light provides a very flexible tool for the production of configurable and flexible confining potentials at the nano- and micro-scale. Sculpted light has been extensively used in diverse fields ranging from optical micromanipulation to quantum atom optics and quantum communication. It enables the production of complex optical potentials.
Optically driven nano and micromachines hold a promise of vast applications in interdisciplinary fields of science. These machines can be driven by versatile optical landscapes and provide applications ranging from microfluidics, drag delivery systems to complex biological systems.
Mechanobiology has become an important and ever-growing field of science that combines biology, engineering, chemistry and physics. It provides invaluable tool to studies of how the application of physical forces influences development, cell differentiation, physiology and disease. Many techniques have been developed throughout the time that enable these studies to contribute to our knowledge of complex biological systems. Optical or light technologies on the nano and micro-scale have enabled unprecedented advances in our understanding of mechanobiology. We will review the new developments in this rich field and point at further developments in this area that could lead to use of these nanotools to a further biomedical research community.
We study far from equilibrium systems through investigating how groups of bacteria behave when other bacteria are present in the system and when barriers are introduced. For these studies we use structured light to fabricate microscopic structures for optical trapping and cell studies using two photon photopolymerization process. Structured light is created using spatial light modulator and correct for wavefront distortions in-situ providing aberration corrected system. This system is used to enable production of simple holographic optical tweezers apparatus with as many as 50 individual foci to create complex 3D microstructures. These structures can induce the collective behaviour of bacteria.
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