The tradeoff between spatial-temporal resolutions and phototoxicity prevents fast 3D super-resolution imaging from living cells, hindering the reveal of the subcellular interactions. Key challenges lie in the quests to optimal excitation and efficient super-resolution. Here, we present a double-ring modulated SPIM design toward an ultrathin uniform light sheet with low side lobes. The combination of IDDR SPIM with our isotropic divide-stages-to-process (ID) networks allows live-cell imaging at isotropic 100 nm resolution with a volume rate up to 17 Hz for thousands of time points.
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