Fast and accurate drug screening can significantly improve the survival rate of cancer patients. Here, we present a new drug screening platform for cancer treatment based on an integrated precision microtome and LiMo microscope. Through this platform, tumor specimens from patients are first sectioned into 100-micron thick tissue slices and cultured for a few days; next, the live tumor tissue array is tested with different anti-cancer drugs for a few weeks and regularly 3D imaged at subcellular resolution, realizing personalized medicine. Our preliminary study on human oral cancer tissue has confirmed the feasibility of the drug screening platform.
Block face imaging is widely used in three-dimensional large biological samples imaging. However, high throughput and excellent optical sectioning cannot be achieved at the same time for imaging such large area. Here, we propose a line-scanning virtual structured modulation method to combine the advantages of optical sectioning and imaging speed in structured modulation microscopy and line-scanning microscopy. We significantly improve signal to noise ratio and throughput compared to wild-filed structured illumination microscopy. Our results also have no residual modulation artifacts. It indicates our method enables to achieve imaging 3D large biological sample with excellent optical sectioning and high throughput.
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