Simultaneous Label-free Autofluorescence Multiharmonic (SLAM) microscopy is a nonlinear multimodal optical imaging technique with sub-micron spatial resolution, enabling 3-D visualization and analysis of live cells, complex in vitro models, and tissues. SLAM microscopy detects NAD(P)H and FAD autofluorescence as well as second and third harmonic generation signals simultaneously from biological samples. It can be used for a wide range of applications in cell-to-clinic pharmaceutical research. To run proof-of-concept, longitudinal, and clinical studies of interest to GSK project teams, the GSK Center for Optical Molecular Imaging (COMI) was established in 2015. Based on promising results from these studies, GSK contracted with spin-out start-up, LiveBx, to design and develop the first portable SLAM microscope, and is currently being used for studies on-site at GSK. In this presentation, major milestones and challenges in translating the SLAM technology from academia to industry and key learnings from this process will be shared from multiple perspectives.
We investigated the potential of Simultaneous Label-free Autofluorescence Multiharmonic (SLAM) microscopy for quantitative evaluation of cisplatin-induced nephrotoxicity in rats. To determine the structural and functional changes associated with cisplatin treatment occurring over a period of time, rats were euthanized at different time points post-treatment (days 2, 6 and 29), and SLAM images were collected from 4% PFA-fixed sagittal kidney sections. Signs of renal tubular injury including hyaline cast formations were detected in SLAM images obtained from day 6 and 29 time points. This study demonstrated the capability of SLAM for visualization and evaluation of cisplatin-induced nephrotoxicity in a label-free manner.
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