Mr. Riley Q. McKeough Profile

Riley Q. McKeough

SPIE Involvement:

Author

Publications (2)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 5 March 2021 Presentation + Paper

Thermal stabilization of nucleic acid nanoparticles (NANPs) using light-assisted drying

Daniel Furr, Phuong Anh Lam, Allison Tran, Riley McKeough, Damian Beasock, Morgan Chandler, Kirill Afonin, Susan Trammell

Proceedings Volume 11659, 1165913 (2021) https://doi.org/10.1117/12.2577763

KEYWORDS: Nanoparticles, Solids, Laser processing, Crystals

Read Abstract +

Cold storage can be challenging and expensive for the transportation and storage of biologics. We are developing a new processing technique, light-assisted drying (LAD), to prepare biologics for anhydrous storage in a trehalose amorphous solid matrix. Nucleic acid nanoparticles (NANPs) are an example of new biological products that require refrigeration. DNA and RNA have emerged as building blocks for versatile biological drugs, called therapeutic nucleic acids (TNAs). NANPs have been developed to simultaneously deliver multiple TNAs and to conditionally activate TNAs and control their immunorecognition. The structural and chemical instability of NANPs over long-term storage at ambient temperatures is a challenge that may hamper broad use of this promising technology. In this work we apply the LAD technique to NANPs. NANPs suspended in a droplet of trehalose solution are irradiated with a near-IR laser to accelerate drying. As water is removed, the trehalose forms a protective matrix. The laser allows for careful control of sample temperature during processing. This is important as NANPs are thermally sensitive. In this study, RNA cubes (a type of NANP) were LAD processed and then stored for 1 month. Damage to LAD-processed NANPs was assessed after storage using gel electrophoresis and compared to unprocessed controls stored at 4°C. The thermal histories of samples were monitored during processing to determine the importance of temperature excursions on NANP viability after processing. The trehalose matrix was characterized using polarized light imaging to determine if crystallization occurred during storage, damaging embedded NANPs. These preliminary studies indicate that LAD processing can stabilize NANPs for dry-state storage at room temperatures.

Proceedings Article | 21 February 2020 Presentation + Paper

Light-assisted drying (LAD) for anhydrous preservation of biologics: using Raman spectroscopy to assess the uniformity of drying in processed samples

Riley McKeough, Daniel Furr, Fan Zhang, Yong Zhang, Susan Trammell

Proceedings Volume 11230, 112300K (2020) https://doi.org/10.1117/12.2546027

KEYWORDS: Proteins, Raman spectroscopy, Solids, Glasses, Humidity, Cameras, Matrices, Near infrared spectroscopy, Laser processing, Photonics

Read Abstract +

Protein-based therapeutics have been developed to treat a range of conditions and assays use immobilized capture proteins for the detection of diseases. A challenge in the development of protein-based products is maintaining the protein in the folded state during processing and storage. The most common method of stabilizing proteins for storage is lyophilization. However, the freeze-drying process remains expensive and many proteins that are lyophilized must be refrigerated or frozen to maintain functionality. Cold storage strategies can be challenging for the transportation of protein-based products and can be difficult or impossible in low resource settings. Recent research has demonstrated that anhydrous, or dry state, preservation in a trehalose amorphous solid matrix offers an alternative to freeze drying for the preservation of biologics. We have previously described a new processing technique, light assisted drying (LAD), to create trehalose preservation matrices. LAD uses illumination by near-infrared laser light to selectively heat water and speeds dehydration of small volume (40 μL) samples. Low end moisture contents (EMC’s) are necessary for storage at supra-zero temperatures and this low water content must be uniform to insure successful long-term storage of embedded biologics. Our previous work has demonstrated the ability of LAD to reach EMCs necessary for storage at elevated temperatures. In this work, Raman spectroscopy is used to assess the trehalose distribution and water content across LAD processed samples. Results indicate that the water content of LAD process samples is uniform. LAD is a promising technique for processing biologics in preparation for anhydrous storage.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years