Interaction of polarized light with tissue samples is an emergent and powerful tool for biomedical diagnosis due to the possibility of providing quantitative information through Stokes parameters and Mueller matrix elements. Division of Focal Plane polarimetric sensing in particular has recently become popular due to advances in technology and commercial availability of microgrid polarizers integrated onto the sensing chip of a camera. After calibration of the system, intensity measures from these sensors can be mapped to the State of Polarization (SoP) of the input. A microscopy setup incorporating DoFP sensing can allow then to obtain Stokes parameters corresponding to a given Field of View (FoV) of a tissue sample. By illuminating with linearly independent SoP in the input, Mueller matrix elements can also be retrieved for the FoV of the sample. Finally, FoV extension to whole-slide Mueller matrix imaging can be achieved through stitching of multiple images taken by means of a motorized XY stage. Validation results for whole-slide Mueller matrix elements of tissue samples are presented.
Interaction of light with biological material constitutes a powerful tool for example in medical diagnosis. For example, the degree of linear polarization (DoLP) allows to differentiate between healthy and cancerous tissue. Polarimetric imaging by means of space-division techniques has become recently popular due to advances in nanotechnology and commercial availability of microgrid polarizers integrated onto the sensing chip of a camera. In order to obtain the linear Stokes parameters from a sample in a single shot, we developed a microscopy setup incorporating a polarized sensor in the imaging plane. Besides, by means of illumination multiplexing with linear States of Polarization in the input, Mueller matrix elements can be retrieved in a single shot. Experimental results for measures of DoLP and AoP as well as Mueller matrix elements over tissue samples are presented.
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