This work is devoted to the substantiation and selection of patients with ovarian tumor for the purpose of conducting expensive molecular genetic studies on genotyping. As diagnostic methods have been used ultraviolet spectrometry samples of blood plasma in the liquid state, infrared spectroscopy middle range (2,5 - 25 microns) dry residue of plasma polarization and laser diagnostic technique of thin histological sections of biological tissues. Obtained results showed that the use of spectrophotometry in the range of 1000-3000 cm-1 allowed to establish quantitative parameters of the plasma absorption rate of blood of patients in the third group in different ranges, which would allow in the future to conduct an express analysis of the patient's condition (procedure screening) for further molecular-genetic typing on BRCA I and II.
The purpose of the study: to conduct laser-spectrophotometric differential diagnosis of blood in postmenopausal patients with benign processes and endometrial cancer. It was analysed on this work the dependings of autocorrelation functionsK[S ( x)] i=2;3;4 ∆ of coordinate distributions of the Stokes vector parameters polarization-inhomogeneous laser object fields of eksocervix native smears and cervical canal wall. It was revealed the behavior of power spectra distribution parameters Stokes vector ( , ) 1,2,3,4 S x y i= of object field cervix. Choice of statistical studies set points 1-4 orders polarized coherent radiation field, transformed as a result of interaction with the oncologic modified biological layers "epithelium - stroma" as a quantitative criterion polarimetric optical differentiation state of human biological tissues was proved. It is established that the increase in the value of birefringence layers of epithelial dysplasia in biological tissues and networks of collagen fibers with adenocarcinoma manifested in the growth of statistical significance since the 3rd order coordinate distributions of polarization of laser images smears and tissue sections cervix.
The purpose of the work is to demonstrate the effectiveness of optical diagnostics of cytological smears of the cervix for the presence of HPV using spectral-polarization methods. 30 cytological smears of the cervix with koilocytosis and 15 samples without koilocytosis were studied. Comparison of the obtained results with the viral load of HPV genomic equivalents in a smear-scraping by the PCR method, it was established that at wavelengths of 405-425 nm, less than 3 HE and 100 thousand cells correspond (clinically insignificant result). In contrast, at wavelengths of 425-440 nm corresponds to more than 5 HE per 100 thousand cells, which is a clinically significant variant (high carcinogenic risk). On the basis of the conducted research, it was established that the use of spectro-polarization research and the fluorescence method allows to increase the accuracy of the selection of patients for an expensive procedure - DNA diagnosis of HPV with a high carcinogenic risk by the standard PCR method.
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