Prof. Satoshi Nishimura Profile

Prof. Satoshi Nishimura

at Jichi Medical Univ

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Proceedings Article | 15 March 2018 Presentation

4K/8K CMOS-based, broad-view, high-resolution, and ultra-rapid in vivo imaging analysis (Conference Presentation)

Satoshi Nishimura

Proceedings Volume 10505, 105050T (2018) https://doi.org/10.1117/12.2286303

KEYWORDS: In vivo imaging, CMOS sensors, Imaging systems, Visualization, Integrated optics, Mechanics, Electronics, Robotics, Virtual reality, Stereoscopy

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We developed 4/8K CMOS sensors, and spinning-disk based multi-color imaging system for living animals. 4/8K pixels, 60fps, and 4ch imaging covered from micro- to macro-scale in time and space, which enabled us to track single cell for long time including diseases progressions. Optical path was enlarged for large CMOS sensors, and pixel numbers. Amount of information per time was increased up to 6TB/1hr, and these system enabled us to track single cell for long time with sub-micro resolutions. We integrated optics, mechanics and electronics into one system, and sample manipulations were supported by robotics and VR (virtual reality). To understand dynamically developing thrombus and vascular responses, which is one of the main components of cardiovascular diseases, we performed intravital vessel visualization. We visualized, acute thrombus formation and chronic inflammatory responses in one views. We revealed platelet behaviors and platelet morphological changes at micro level, and malfunctioning at macro organ levels in real-time manners. Inflammatory reactions in hours are simultaneously evaluated by single imaging modality.

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