Prof. Singaravelu Ganesan Profile

Prof. Singaravelu Ganesan

Professor at Anna Univ Chennai

SPIE Involvement:

Conference Program Committee | Author

Publications (55)

Proceedings Article | 13 March 2024 Poster + Paper

Raman and fluorescence spectroscopic characterization of pteridines in the blood of normal subjects and oral cancer patients

Elumalai Brindha, Ramu Rajasekaran, Ganesan Bharanidharan, Biniyam Girma Manaye, Prakasarao Aruna, Singaravelu Ganesan

Proceedings Volume 12836, 128360I (2024) https://doi.org/10.1117/12.3010048

KEYWORDS: Cancer, Raman spectroscopy, Fluorescence, Blood, Plasma, Biological samples, Spectroscopy, Fluorescence spectroscopy, Diseases and disorders, Cancer detection

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Proceedings Article | 13 March 2024 Poster + Paper

Optical characterization of riboflavin as a potential biomarker in the diagnosis of cervical cancer

Ramu Rajasekaran, Elumalai Brindha, Ganesan Bharanidharan, Biniyam Girma Manaye, Prakasarao Aruna, Singaravelu Ganesan

Proceedings Volume 12836, 128360H (2024) https://doi.org/10.1117/12.3010046

KEYWORDS: Cervical cancer, Fluorescence, Cancer, Fluorophores, Principal component analysis, Fluorescence intensity, Emission wavelengths, Statistical analysis, Mode conditioning cables, Matrices

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Proceedings Article | 5 March 2021 Paper

Discrimination of urinary tract infection bacteria using redox imaging

Anandh Sundaramoorthy, Einstein Gnanatheepam, Kailasha Purushothaman, Aruna Prakasa Rao, Bharanidaran Ganesan, Ganesan Singaravelu

Proceedings Volume 11648, 116480L (2021) https://doi.org/10.1117/12.2583092

KEYWORDS: Bacteria, Machine learning, Luminescence, Multiphoton fluorescence microscopy, Deconvolution, Biological research

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Proceedings Article | 5 March 2021 Paper

Quantification of tryptophan in different layers of oral cancer tissue using fluorescence polarization technique

Einstein Gnanatheepam, Anandh Sundaramoorthy, Kanniyappan Udayakumar, Dornadula Koteeswaran, Singaravelu Ganesan, Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 11636, 116360G (2021) https://doi.org/10.1117/12.2583352

KEYWORDS: Cancer, Polarization, Tissues, Luminescence, Tissue optics, Statistical analysis, Multiple scattering, Light scattering, Fluorescence spectroscopy, Diagnostics

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Proceedings Article | 5 March 2021 Paper

Multiphoton confocal imaging of mammalian cells in presence of Zinc Nitride nanoparticle

Prabha Soundharraj, Durgalakshmi Dhinasekaran, Ganesan Singaravelu, Anandh Sundaramoorthy, Aruna Prakasa Rao

Proceedings Volume 11648, 116480O (2021) https://doi.org/10.1117/12.2583362

KEYWORDS: Nanoparticles, Luminescence, Zinc, Multiphoton fluorescence microscopy, Tissues, Nanomaterials, Fluorescence resonance energy transfer, Signal to noise ratio, Resonance enhancement, Resolution enhancement technologies

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Proceedings Article | 21 February 2020 Presentation + Paper

Correlation of metabolites in saliva and in vivo tissue of oral cancer patients based on fluorescence spectral deconvolution

Raja Pappu, Yuvaraj Manoharan, Einstein Gnanatheepam, Sangeetha Ramamoorthy, Prakasarao Aruna, Jayachandran Sadaksharam, Singaravelu Ganesan

Proceedings Volume 11234, 112341D (2020) https://doi.org/10.1117/12.2545662

KEYWORDS: Luminescence, Tissues, Cancer, Deconvolution, Tissue optics, In vivo imaging, Fluorescence spectroscopy, Diagnostics, Spectroscopy, Monochromators

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Proceedings Article | 14 February 2020 Presentation + Paper

Live cell metabolic imaging of cancer cell lines using multiphoton fluorescence polarization

Einstein Gnanatheepam, Anandh Sundaramoorthy, Bharanidharan Ganesan, Kailasha Purushothaman, Durgalakshmi Dhinasekaran, Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 11244, 112441B (2020) https://doi.org/10.1117/12.2545847

KEYWORDS: Cancer, Luminescence, Polarization, Anisotropy, Deconvolution, Image segmentation, Multiphoton fluorescence microscopy, Live cell imaging, Image processing, Molecules

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Proceedings Article | 4 March 2019 Paper

Evaluation of variations in plasma collagen NADH and flavin in pre and post treated breast cancer patients using native fluorescence spectroscopy

Chithra K., Vijayaraghavan S., Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 10873, 1087308 (2019) https://doi.org/10.1117/12.2509907

KEYWORDS: Breast cancer, Collagen, Plasma, Cancer, Luminescence, Fluorescence spectroscopy, Oncology, Surgery, Blood, Statistical analysis

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Proceedings Article | 21 February 2018 Paper

Stokes shift spectroscopy for the early diagnosis of epithelial precancers in DMBA treated mouse skin carcinogenesis

Ebenezar Jeyasingh, Ganesan Singaravelu, Aruna Prakasarao

Proceedings Volume 10489, 1048917 (2018) https://doi.org/10.1117/12.2287486

KEYWORDS: Skin, Tissues, Collagen, Spectroscopy, Tumors, Cancer, Diagnostics, Tissue optics, Statistical analysis

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Proceedings Article | 23 March 2017 Paper

Polarized Raman spectroscopic characterization of normal and oral cancer blood plasma

Rekha Pachaiappan, Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 10054, 100541F (2017) https://doi.org/10.1117/12.2255600

KEYWORDS: Data analysis, Raman spectroscopy, Cancer, Blood, Plasma, Spectroscopy, Artificial neural networks, Molecules, Proteins, Tumors

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Proceedings Article | 3 March 2017 Presentation + Paper

Intrinsic fluorescence of protein in turbid media using empirical relation based on Monte Carlo lookup table

Gnanatheepam Einstein, Kanniyappan Udayakumar, Prakasarao Aruna, Singaravelu Ganesan

Proceedings Volume 10063, 100630N (2017) https://doi.org/10.1117/12.2255883

KEYWORDS: Luminescence, Proteins, Monte Carlo methods, Tissues, Diagnostics, Spectroscopy, Oncology, Error analysis, Fluorescence spectroscopy, Tissue optics, Biomedical optics

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Proceedings Article | 17 February 2017 Paper

Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) in the discrimination of normal and oral cancer blood plasma

Rekha Pachaiappan, Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 10060, 100601C (2017) https://doi.org/10.1117/12.2255605

KEYWORDS: Cancer, Blood, Plasma, FT-IR spectroscopy, Artificial neural networks, Diagnostics, Data modeling, Statistical analysis, Infrared radiation, Spectrophotometry, Absorption, Diamond

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Proceedings Article | 17 February 2017 Paper

Quantification of hemoglobin and its derivatives in oral cancer diagnosis by diffuse reflectance spectroscopy

Udayakumar Kaniyappan, Einstein Gnanatheepam, Prakasarao Aruna, Koteeswaran Dornadula, Singaravelu Ganesan

Proceedings Volume 10060, 100601F (2017) https://doi.org/10.1117/12.2255874

KEYWORDS: Tissues, Diffuse reflectance spectroscopy, Cancer, Tissue optics, Reflectance spectroscopy, Integrating spheres, Optical spectroscopy, Spectroscopy, Statistical analysis, Oxygen, Diagnostics, Absorbance, Reflectivity

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Proceedings Article | 17 February 2017 Paper

Characterization and classification of oral tissues using excitation and emission matrix: a statistical modeling approach

Udayakumar Kanniyappan, Einstein Gnanatheepaminstein, Aruna Prakasarao, Koteeswaran Dornadula, Ganesan Singaravelu

Proceedings Volume 10060, 100601E (2017) https://doi.org/10.1117/12.2255872

KEYWORDS: Cancer, Statistical analysis, Tissues, Fluorescence spectroscopy, Diagnostics, Tissue optics, Optical spectroscopy, Luminescence, Data modeling, Molecules

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Proceedings Article | 17 February 2017 Presentation + Paper

Study of anti-cancer effects of chemotherapeutic agents and radiotherapy in breast cancer patients using fluorescence spectroscopy

Chithra K., Vijayaraghavan S., Aruna Prakasarao, Ganesan Singaravelu

Proceedings Volume 10060, 100600L (2017) https://doi.org/10.1117/12.2255814

KEYWORDS: Fluorescence spectroscopy, Cancer, Breast cancer, Radiotherapy, Luminescence, Blood, Plasma, Biological research, Spectroscopy, Calcium, Bromine

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Proceedings Article | 17 February 2017 Paper

Oral cancer detection based on fluorescence polarization of blood plasma at excitation wavelength 405 nm

Rekha Pachaiappan, Aruna Prakasarao, Yuvaraj Manoharan, Koteeswaran Dornadula, Ganesan Singaravelu

Proceedings Volume 10060, 100601D (2017) https://doi.org/10.1117/12.2255718

KEYWORDS: Luminescence, Cancer, Fluorescence spectroscopy, Polarization, Blood, Plasma, Artificial neural networks, Spectroscopy, Anisotropy, Tissues, Diagnostics

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Proceedings Article | 14 February 2017 Presentation + Paper

Raman spectroscopic characterization of urine of normal and cervical cancer subjects

Raja Pappu, Aruna Prakasarao, Koteeswaran Dornadula, Ganesan Singaravelu

Proceedings Volume 10054, 1005404 (2017) https://doi.org/10.1117/12.2255878

KEYWORDS: Raman spectroscopy, Cancer, Cervical cancer, Helium neon lasers, Cervix, Statistical analysis, Spectroscopy, Urea, Molecules, Diagnostics, Biopsy

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Proceedings Article | 14 February 2017 Presentation + Paper

High wavenumber Raman spectroscopic characterization of normal and oral cancer using blood plasma

Rekha Pachaiappan, Aruna Prakasarao, Murugesan Suresh Kumar, Ganesan Singaravelu

Proceedings Volume 10054, 1005402 (2017) https://doi.org/10.1117/12.2255602

KEYWORDS: Raman spectroscopy, Blood, Plasma, Cancer, Principal component analysis, Spectroscopy, Statistical analysis, Tissues, In vivo imaging, Proteins

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SPIE Journal Paper | 15 June 2016

Plasmonic phototherapy using gold nanospheres and gold nanorods irradiated with light-emitting diodes

Gananathan Poorani, Aruna Prakasa Rao, Ganesan Singaravelu, Elanchezhiyan Manickan

JNP, Vol. 10, Issue 02, 026027, (June 2016) https://doi.org/10.1117/12.10.1117/1.JNP.10.026027

KEYWORDS: Nanorods, Gold, Light emitting diodes, Satellite navigation systems, Plasmonics, Phototherapy, Nanoparticles, Particles, Cancer, Absorption

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Proceedings Article | 22 April 2016 Presentation + Paper

Photo thermal efficacy of green light emitting diode and gold nano spheres for malignancy

Poorani Gananathan, Aruna Prakasa Rao, Singaravelu Ganesan, Elanchezhiyan Manickan

Proceedings Volume 9722, 97220U (2016) https://doi.org/10.1117/12.2213167

KEYWORDS: Light emitting diodes, Gold, Nanoparticles, Light sources, Cell death, Green light emitting diodes, Optical spheres, Light, Plasmonics, Particles, Tumors, Absorption

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Proceedings Article | 7 March 2016 Paper

An empirical formula based on Monte Carlo simulation for diffuse reflectance from turbid media

Einstein Gnanatheepam, Prakasa Rao Aruna, Singaravelu Ganesan

Proceedings Volume 9703, 97031V (2016) https://doi.org/10.1117/12.2213018

KEYWORDS: Monte Carlo methods, Diffuse reflectance spectroscopy, Optimization (mathematics), Scattering, Optical properties, Natural surfaces, Tissues, Biological research, Biomedical optics, Liquids, Diagnostics, Oncology

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Proceedings Article | 7 March 2016 Paper

The effect of Stokes shift in the discrimination of urine of cervical cancer from normal subjects

Ramu Rajasekaran, Elumalai Brindha, Prakasa Rao Aruna, Dornadula Koteeswaran, Singaravelu Ganesan

Proceedings Volume 9703, 97031Q (2016) https://doi.org/10.1117/12.2213178

KEYWORDS: Fluorescence spectroscopy, Cervical cancer, Tissues, Spectroscopy, Luminescence, Diagnostics, Principal component analysis, Microfluidics, Cancer, Signal to noise ratio, Biopsy, Biological research

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Proceedings Article | 7 March 2016 Presentation + Paper

Steady state fluorescence spectroscopic characterization of normal and diabetic urine at selective excitation wavelength 280 nm

Anjana Kesavan, Rekha Pachaiappan, Prakasa Rao Aruna, Singaravelu Ganesan

Proceedings Volume 9703, 97031H (2016) https://doi.org/10.1117/12.2212625

KEYWORDS: Data processing, Luminescence, Statistical analysis, Fluorescence spectroscopy, Spectroscopy, Statistical methods, Cancer, Monochromators, Data acquisition, Light

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Proceedings Article | 7 March 2016 Paper

Raman spectroscopy of bio fluids: an exploratory study for oral cancer detection

Elumalai Brindha, Ramu Rajasekaran, Prakasarao Aruna, Dornadula Koteeswaran, Singaravelu Ganesan

Proceedings Volume 9703, 97031T (2016) https://doi.org/10.1117/12.2212684

KEYWORDS: Cancer, Raman spectroscopy, Blood, Tissue optics, Statistical analysis, Optical spectroscopy, Spectroscopy, Diagnostics, Oncology, Biopsy, Tissues, Molecules, Plasma, Biological research

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Proceedings Article | 7 March 2016 Paper

Fluorescence anisotropy characterization of urine in the diagnosis of cancer

Ramu Rajasekaran, Elumalai Brindha, Shanmugam Sivabalan, Prakasa Rao Aruna, Dornadula Koteeswaran, Singaravelu Ganesan

Proceedings Volume 9703, 97031P (2016) https://doi.org/10.1117/12.2212618

KEYWORDS: Cancer, Luminescence, Polarization, Fluorescence spectroscopy, Diagnostics, Anisotropy, Oncology, Spectroscopy, Fluorescence anisotropy, Cervical cancer, Statistical analysis, Tissue optics

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Proceedings Article | 7 March 2016 Presentation + Paper

Study on discrimination of oral cancer from normal using blood plasma based on fluorescence steady and excited state at excitation wavelength 280 nm

Pachaiappan Rekha, Prakasa Rao Aruna, Singaravelu Ganesan

Proceedings Volume 9703, 97031F (2016) https://doi.org/10.1117/12.2213098

KEYWORDS: Blood, Plasma, Cancer, Luminescence, Fluorescence spectroscopy, Tissues, Statistical analysis, Spectroscopy, Proteins, Collagen

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Proceedings Article | 5 March 2015 Paper

Discrimination of premalignant conditions of oral cancer using Raman spectroscopy of urinary metabolites

Brindha Elumalai, Ramu Rajasekaran, Prakasarao Aruna, Dornadula Koteeswaran, Singaravelu Ganesan

Proceedings Volume 9318, 93180J (2015) https://doi.org/10.1117/12.2078029

KEYWORDS: Raman spectroscopy, Cancer, Spectroscopy, Urea, Ovarian cancer, Principal component analysis, Diagnostics, Statistical analysis, Tissue optics, Optical spectroscopy

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Proceedings Article | 24 March 2014 Paper

Photoirradiation study of gold nanospheres and rods in Vero and Hela cell lines

Poorani Gananathan, Prakasarao Aruna, Singaravelu Ganesan, Manickan Elanchezhiyan

Proceedings Volume 8955, 89551T (2014) https://doi.org/10.1117/12.2041933

KEYWORDS: Gold, Nanoparticles, Absorption, Particles, Light emitting diodes, Light sources, Cell death, Tissues, Toxicity, Nanorods

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SPIE Journal Paper | 19 March 2014 Open Access

Steady-state and time-resolved fluorescence spectroscopic characterization of urine of healthy subjects and cervical cancer patients

Ramu Rajasekaran, Prakasa Rao Aruna, Dornadula Koteeswaran, Ganesan Bharanidharan, Munusamy Baludavid, Singaravelu Ganesan

JBO, Vol. 19, Issue 03, 037003, (March 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.3.037003

KEYWORDS: Cancer, Luminescence, Cervical cancer, Time resolved spectroscopy, Fluorescence spectroscopy, Spectroscopy, Statistical analysis, Tissues, Diagnostics, Oncology

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Proceedings Article | 17 March 2014 Paper

Noninvasive diagnosis of oral cancer by Stokes shift spectroscopy

Jeyasingh Ebenezar, Singaravelu Ganesan, Prakasrao Aruna, Radhakrishnan Muralinaidu

Proceedings Volume 8940, 89400L (2014) https://doi.org/10.1117/12.2036798

KEYWORDS: Cancer, Collagen, Tissues, Luminescence, Spectroscopy, Diagnostics, Fluorescence spectroscopy, Monochromators, Statistical analysis, In vivo imaging

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Proceedings Article | 19 March 2013 Paper

Steady state and time-resolved fluorescence spectroscopic characterisation of normal and cancerous urine

Ramu Rajasekaran, Prakasa Rao Aruna, Munusamy Balu David, Dornadula Koteeswaran, Kulandaivel Muthuvelu, Rai R., Singaravelu Ganesan

Proceedings Volume 8577, 857707 (2013) https://doi.org/10.1117/12.2006086

KEYWORDS: Cancer, Luminescence, Time resolved spectroscopy, Fluorescence spectroscopy, Spectroscopy, Principal component analysis, Statistical analysis, Tissues, Diagnostics, Radiation oncology

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SPIE Journal Paper | 21 September 2012 Open Access

Noninvasive fluorescence excitation spectroscopy for the diagnosis of oral neoplasia in vivo

Jeyasingh Ebenezar, Singaravelu Ganesan, Prakasarao Aruna, Radhakrishnan Muralinaidu, Kannan Renganathan, Thillai Saraswathy

JBO, Vol. 17, Issue 9, 097007, (September 2012) https://doi.org/10.1117/12.10.1117/1.JBO.17.9.097007

KEYWORDS: Tissues, Luminescence, Diagnostics, Spectroscopy, Fluorescence spectroscopy, In vivo imaging, Absorption, Cancer, Tissue optics, Statistical analysis

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Proceedings Article | 17 February 2011 Paper

Site-dependant redox ratio in healthy oral cavity

Sivabalan Shanmugam, Dornadula Koteeswaran, Prakasarao Aruna, Singaravelu Ganesan

Proceedings Volume 7895, 78950M (2011) https://doi.org/10.1117/12.873713

KEYWORDS: Luminescence, Cancer, Tissues, Tongue, Fluorescence spectroscopy, Tissue optics, In vivo imaging, Diagnostics, Cervical cancer, Spectroscopy

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Proceedings Article | 17 February 2011 Paper

Synchronous luminescence spectroscopic characterization of blood elements of normal and patients with cervical cancer

K. Muthuvelu, Sivabalan Shanmugam, Dornadula Koteeswaran, S. Srinivasan, P. Venkatesan, Prakasarao Aruna, Singaravelu Ganesan

Proceedings Volume 7895, 78950F (2011) https://doi.org/10.1117/12.876164

KEYWORDS: Cervical cancer, Luminescence, Blood, Spectroscopy, Fluorescence spectroscopy, Laser sintering, Cancer, Tissues, Diagnostics, Statistical analysis

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Proceedings Article | 12 February 2010 Paper

Stokes shift spectroscopy for breast cancer diagnosis

Ebenezar Jeyasingh, Aruna Prakashrao, Ganesan Singaravelu

Proceedings Volume 7561, 75610B (2010) https://doi.org/10.1117/12.840196

KEYWORDS: Tissues, Breast, Luminescence, Collagen, Breast cancer, Diagnostics, Spectroscopy, Absorption, Fluorescence spectroscopy, Monochromators

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Proceedings Article | 12 February 2010 Paper

Diffuse reflectance spectroscopy of pre- and post-treated oral submucous fibrosis: an in vivo study

S. Sivabalan, C. Ponranjini Vedeswari, S. Jayachandran, D. Koteeswaran, C. Pravda, P. Aruna, S. Ganesan

Proceedings Volume 7561, 75610T (2010) https://doi.org/10.1117/12.843090

KEYWORDS: Diffuse reflectance spectroscopy, Tissue optics, In vivo imaging, Mouth, Reflectivity, Diagnostics, Cancer, Optical fibers, Spectroscopy, Collagen

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SPIE Journal Paper | 1 January 2010 Open Access

In vivo native fluorescence spectroscopy and nicotinamide adinine dinucleotide/flavin adenine dinucleotide reduction and oxidation states of oral submucous fibrosis for chemopreventive drug monitoring

Sivabalan Sivabalan, C. Ponranjini Vedeswari, S. Jayachandran, D. Koteeswaran, C. Pravda, Prakashrao Aruna, Singaravelu Ganesan

JBO, Vol. 15, Issue 01, 017010, (January 2010) https://doi.org/10.1117/12.10.1117/1.3324771

KEYWORDS: Luminescence, Fluorescence spectroscopy, Collagen, In vivo imaging, Tissues, Statistical analysis, Mouth, Cancer, Tongue, Oxidation

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Proceedings Article | 1 May 2002 Paper

Native fluorescence spectroscopy of cervical tissues: classification by different statistical methods

Singaravelu Ganesan, Nammalver Vengadesan, Thalaimuthu Anbupalam, Srinivasan Hemamalini, Prakasa Aruna, P. Karkuzhali

Proceedings Volume 4613, (2002) https://doi.org/10.1117/12.465255

KEYWORDS: Tissues, Statistical analysis, Luminescence, Fluorescence spectroscopy, Statistical methods, Proteins, Diagnostics, Biopsy, Principal component analysis, Cervical cancer

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Proceedings Article | 1 May 2002 Paper

Characterization of cervical normal and abnormal tissues by synchronous luminescence spectroscopy

Nammalver Vengadesan, Thalaimuthu Anbupalam, Srinivasan Hemamalini, Jeyasingh Ebenezar, Kulandhaivel Muthvelu, D. Koteeswaran, Prakasa Aruna, Singaravelu Ganesan

Proceedings Volume 4613, (2002) https://doi.org/10.1117/12.465247

KEYWORDS: Tissues, Luminescence, Statistical analysis, Spectroscopy, Principal component analysis, Fluorescence spectroscopy, Diagnostics, Monochromators, Collagen, Biopsy

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Proceedings Article | 1 May 2002 Paper

Ultraviolet emission and excitation fluorescence spectroscopic characterization of DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation

Prakasa Aruna, Srinivasan Hemamalini, Jeyasingh Ebenezar, Singaravelu Ganesan

Proceedings Volume 4613, (2002) https://doi.org/10.1117/12.465227

KEYWORDS: Skin, Luminescence, Tissues, Fluorescence spectroscopy, Ultraviolet radiation, Proteins, In vivo imaging, Tumor growth modeling, Spectroscopy, Tissue optics

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Proceedings Article | 1 May 2002 Paper

In-vivo characterization of endogenous porphyrin fluorescence from DMBA-treated Swiss Albino mice skin carcinogenesis for measuring tissue transformation

Singaravelu Ganesan, Jeyasingh Ebenezar, Srinivasan Hemamalini, Prakasa Aruna

Proceedings Volume 4613, (2002) https://doi.org/10.1117/12.465237

KEYWORDS: Skin, Tissues, Luminescence, Tumors, Fluorescence spectroscopy, In vivo imaging, Spectroscopy, Cancer, Skin cancer, Tumor growth modeling

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Proceedings Article | 26 October 2001 Paper

In-vivo pharmacokinetics of delta-ALA induced PPIX during photodynamic therapy on mice tumor model

Diagaradjane Parmeswearan, Prakasa Aruna, Pradeep Gupta, Singaravelu Ganesan

Proceedings Volume 4433, (2001) https://doi.org/10.1117/12.446514

KEYWORDS: Photodynamic therapy, Tumors, Tissues, Luminescence, Mathematical modeling, In vivo imaging, Diffusion, Data modeling, Laser therapeutics, Cancer

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In the recent years (delta) -aminolevulinic acid ((delta) -ALA) a precursor for the endogenous production of protoporphyrin IX (PPIX) has gained importance in the Photodynamic Therapy (PDT) of superficial and early-stage cancers. Though (delta) -ALA is present naturally in the cells, systemic administration of exogenous (delta) -ALA leads to the production of intracellular endogenous PP IX in both the tumor and the normal cells, but with varying concentration. However, the PPIX is accumulated more in the tumor tissues as the fast growing tumor cells take up the administered (delta) -ALA more than the normal cells. As the therapeutic efficacy of PDT is dependent on the post (delta) -ALA incubation time, at which the tumor to normal ratio of the PPIX concentration is high, the concentration of the PPIX in the normal and the tumor site were estimated using fluorescence spectroscopy. However, the estimation of the PPIX concentration during/after PDT is mandatory, as the PDT dosimetry is dependent on the sensitizer concentration at the target of interest. The observed variation in the concentration of PPIX in the tumor site with respect to the unexposed normal surrounding tissues, may be attributed to the diffusion of PPIX from the surrounding normal tissues to the tumor site, across the concentration gradient. Based on this a mathematical model has been proposed, to estimate the rate parameter for the diffusion of PPIX from the surrounding normal tissues in to the tumor tissue (K_m), due to photobleaching during PDT at two different fluence. The K_m value at two different fluences, 57.6 and 36 J/cm2 are estimated as 5.444+/- 1.186 and 3.221+/- 0.957, respectively. Further, the rate parameter for the cleavage and efflux of (delta) -ALA (K₁), and the rate parameter for the evasion of the PPIX from the tumor tissues during PDT (K_t), were also estimated by fitting the experimental data to the developed mathematical model. The estimated parameters will be utilized to estimate the exact concentration of PPIX in the tumor tissues for a better PDT efficacy.

Proceedings Article | 24 April 2001 Paper

Kinetics and comparison of delta-aminolevulinic-acid-induced endogenous protoporphyrin-IX in single cell by steady state and multiphoton fluorescence imaging

Singaravelu Ganesan, Masilamani Elangovan, Ammasi Periasamy

Proceedings Volume 4262, (2001) https://doi.org/10.1117/12.424563

KEYWORDS: Luminescence, Tumors, Single photon, Photodynamic therapy, Multiphoton fluorescence microscopy, Optical filters, Light sources, Oxygen, Absorption, Microscopes

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Photodynamic Therapy has emerged as a new modality in the treatment of various nonmalignant and malignant diseases. It involves the systemic administration of tumor specific photo-sensitizers with the subsequent application of visible light. This combination causes the generation of cytotoxic species, which damage sensitive targets, producing cell injury and tumor destruction. Although, photofrin is the only photosensitizer currently approved for PDT and tumor detection, its concomitant cutaneous photosensitization poses a significant problem. Hence, δ-aminoleuvulinic acid (δ-ALA) a precursor for the endogenous production of Protoporphyrin IX, through heme biosynthesis pathway, has gained significant importance in the Photodynamic Therapy. Though δ-ALA is present naturally in the cells, exogenous δ-ALA helps to synthesis more of PpIX in the tumor cells, as the fast growing tumor cells take up the administered δ-ALA more than the normal cells. Based on these facts, many invasive studies have been reported on the kinetics of δ-ALA at cellular level by chemical extraction of PpIX from the cells. In the present study we have studied the kinetics of δ-ALA induced PpIX fluorescence from Hela cells by perchloric/Methanol extraction method. However, the amount of PpIX synthesized in the cells at different point of incubation time by noninvasive methods has not been reported. Hence we have also used a noninvasive technique of measuring the kinetics δ-ALA induced PPIX fluorescence from Hela, an epithelial cell derived from human cervical cancer by both single photon (steady state) and multi photon excitation. From the studies it is observed that the δ-ALA induced PpIX is more at 2 hours incubation time for 2 mM of δ-ALA concentration. Further, it is observed that with steady state fluorescence imaging method, the excitation light itself cause the Photodynamic damage, due to the prolonged exposure of the cells than in multi photon excitation, leading to the rounding of cells. This may be due to the activation of PpIX in cells by the excitation light source.

Proceedings Article | 16 April 2001 Paper

Optical biopsy of normal and cancerous cervical tissues by native fluorescence spectroscopy

Nammalver Vengadesan, K. Muthuvelu, Thalaimuthu Anbupalam, S. Madhuri, Diagaradjane Parmeswearan, Prakasa Aruna, Singaravelu Ganesan

Proceedings Volume 4261, (2001) https://doi.org/10.1117/12.424530

KEYWORDS: Tissues, Statistical analysis, Luminescence, Fluorescence spectroscopy, Cancer, Cervical cancer, Biopsy, Tissue optics, Collagen, Principal component analysis

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Proceedings Article | 8 May 2000 Paper

Characterization of normal and native oral cancerous tissues by infrared spectroscopy

Nammalver Vengadesan, D. Koteeswaran, Prakasa Aruna, K. Muthuvelu, Singaravelu Ganesan

Proceedings Volume 3918, (2000) https://doi.org/10.1117/12.384954

KEYWORDS: Tissues, Absorption, Cancer, Spectroscopy, Infrared spectroscopy, FT-IR spectroscopy, Proteins, Mid-IR, Lung cancer, Physics

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Proceedings Article | 19 April 2000 Paper

Characterization of human neoplastic and normal oral tissues by visible excitation and emission fluorescence spectroscopy

D. Koteeswaran, Nammalver Vengadesan, Prakasa Aruna, K. Muthuvelu, S. Bharghavi, V. Gowri, Singaravelu Ganesan

Proceedings Volume 3917, (2000) https://doi.org/10.1117/12.382745

KEYWORDS: Tissues, Luminescence, Cancer, Fluorescence spectroscopy, Visible radiation, Tumors, Spectroscopy, Diagnostics, Emission spectroscopy, Chlorine

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Proceedings Article | 6 July 1999 Paper

Photodynamic activity on human erythrocytes by a newly synthesized sensitizer--trithia sapphyrin sulfonate: a preliminary report

Diagaradjane Parmeswearan, Singaravelu Ganesan, K. Thanigaivelu, Simi Pushpan, A. Srinivasan, M. Ravi Kumar, T. Chandrashekar

Proceedings Volume 3592, (1999) https://doi.org/10.1117/12.351511

KEYWORDS: Absorption, Molecules, Photodynamic therapy, Environmental sensing, Luminescence, Tissues, Oxygen, Sulfur, Nitrogen, Diffusion

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Proceedings Article | 19 May 1999 Paper

Comparison of bond strength and surface morphology of dental enamel for acid and Nd-YAG laser etching

Diagaradjane Parmeswearan, Singaravelu Ganesan, P. Ratna, D. Koteeswaran

Proceedings Volume 3593, (1999) https://doi.org/10.1117/12.348332

KEYWORDS: Teeth, Laser bonding, Etching, Laser dentistry, Nd:YAG lasers, Scanning electron microscopy, Photography, Laser energy, Interfaces, Adhesives

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Proceedings Article | 17 May 1999 Paper

Native fluorescence characteristics of blood plasma during rat liver regeneration

Singaravelu Ganesan, S. Madhuri, V. Anuradha, A. Namasivayam, Diagaradjane Parmeswearan

Proceedings Volume 3599, (1999) https://doi.org/10.1117/12.348376

KEYWORDS: Luminescence, Blood, Plasma, Liver, Tissues, Tissue optics, Visible radiation, Injuries, Cancer, Animal model studies

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Proceedings Article | 17 May 1999 Paper

Native fluorescence characterization of human liver abnormalities

Singaravelu Ganesan, S. Madhuri, Prakasa Aruna, S. Suchitra, T. Srinivasan

Proceedings Volume 3599, (1999) https://doi.org/10.1117/12.348380

KEYWORDS: Liver, Luminescence, Blood, Plasma, Fluorescence spectroscopy, Human subjects, Medicine, Biology, Complex systems, Molecules

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Proceedings Article | 18 August 1997 Paper

Native cellular fluorescence characteristics of normal and malignant epithelial cells from human larynx

Diagaradjane Parmeswearan, Singaravelu Ganesan, R. Nalini, Prakasa Aruna, V. Veeraganesh, Robert Alfano

Proceedings Volume 2979, (1997) https://doi.org/10.1117/12.280222

KEYWORDS: Luminescence, Tissues, Fluorescence spectroscopy, Diagnostics, Oncology, Breast, Ultraviolet radiation, Tissue optics, Tumors, Colon

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Proceedings Article | 2 May 1997 Paper

Ultraviolet fluorescence spectroscopy of blood plasma in the discrimination of cancer from normal

S. Madhuri, Prakasa Aruna, M. Summiya Bibi, V. Gowri, D. Koteeswaran, Singaravelu Ganesan

Proceedings Volume 2982, (1997) https://doi.org/10.1117/12.273649

KEYWORDS: Blood, Plasma, Luminescence, Tissues, Cancer, Fluorescence spectroscopy, Ultraviolet radiation, Proteins, Plasma spectroscopy, Diagnostics

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Proceedings Article | 5 April 1996 Paper

Optical biopsy fiber-based fluorescence spectroscopy instrumentation

Alvin Katz, Singaravelu Ganesan, Yuanlong Yang, Gui Tang, Yury Budansky, Edward Celmer, Howard Savage, Stimson Schantz, Robert Alfano

Proceedings Volume 2679, (1996) https://doi.org/10.1117/12.237579

KEYWORDS: Tissues, Breast, Luminescence, Fluorescence spectroscopy, Diagnostics, In vivo imaging, Biopsy, Ultraviolet radiation, Tissue optics, Tumors

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Proceedings Article | 5 April 1996 Paper

Autofluorescence from tissue as a marker for carcinoma

Singaravelu Ganesan, V. Masilamani, J. Nambi, Gowri Nambi, D. Koteeswaran

Proceedings Volume 2679, (1996) https://doi.org/10.1117/12.237588

KEYWORDS: Tissues, Cancer, Tumors, Luminescence, Nitrogen lasers, In vitro testing, Proteins, Biological research, Laser tissue interaction, In vivo imaging

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