Owing to its high resolution, sensitivity, imaged field of view, and frame rate acquisition, Digital Holographic Microscopy (DHM) stands out among the Quantitative phase imaging (QPI) techniques to reconstruct high-resolution phase images from micrometer-sized samples, providing information about the sample’s topography and refractive index. Despite the successful performance of DHM systems, their applicability to in-situ clinical research has been partially hampered by the need for a standard phase reconstruction algorithm that provides quantitative phase distributions without any phase distortion. This invited talk overviews the current advances in computational DHM reconstruction approaches from semi-heuristic to learning-based approaches.
Polarization-sensitive microscopy has been a hot topic in biology over the last decades since many biological samples, including cancer cells and tendons, are intrinsically birefringent. Among its different applications, polarization-sensitive microscopy has enabled the detection and classification of diseases in biological samples by enabling the analysis of their polarimetric properties. In this contribution, we present an apparatus and method to estimate the Mueller matrix of microscopic samples using intensity-based images recorded from a bright-field microscope. We have validated the proposed technique using standard linear polarizers. The hallmark characteristics of our technique are its simplicity and efficiency, being suitable for analyzing biological and other birefringent samples.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.