In this talk, I introduce high-throughput intelligence-powered image-activated cell sorting or an “imaging” version of fluorescence-activated cell sorting [Cell 175, 1 (2018)] that realizes real-time image-based intelligent cell sorting at an unprecedented rate of ~100 events per second. This technology integrates high-speed cell microscopy, focusing, and sorting methods on rapid hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. I present two unique applications in microbiology and hematology enabled by the technology and discuss how technology enables machine-based scientific discovery in diverse biological and medical sciences.
I present a newly developed machine intelligence technology called “Intelligent Image-Activated Cell Sorting” [Cell 175, 1 (2018)] that achieves real-time fluorescence-image-based intelligent cell sorting at a high throughput of ~100 events per second. This technology builds on a unique integration of high-throughput cell microscopy, focusing, and sorting techniques on a unique software-hardware platform and hence performs fully automated operation for data acquisition, data processing, decision making, and actuation. Also, I introduce the technology’s broad utility to addressing a fundamental biological question that cannot be answered by conventional technologies – how molecular architectures of cells are connected with their physiological functions.
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