Ms. Thien-An A. Nguyen Profile

Thien-An A. Nguyen

Optical Scientist

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Publications (7)

Proceedings Article | 6 March 2023 Presentation + Paper

Amyloid beta and tau protein metabolic changes detected by visible resonance Raman scattering microscopy in Alzheimer's disease mouse model

Lingyan Shi, Yan Zhou, Binlin Wu, Ke Zhu, Shengjia Zhang, Susie Boydston-White, Lin Zhang, Zhe Pei, Thien An Nguyen, Cheng-Hui Liu, Robert Alfano

Proceedings Volume 12373, 123730B (2023) https://doi.org/10.1117/12.2652913

KEYWORDS: Proteins, Raman spectroscopy, Brain tissue, Brain, Biological samples, Alzheimer disease, Tissues, Thalamus, Vibration, Spectroscopy

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Alzheimer’s disease (AD) pathogenesis is widely believed to be associated with the production and deposition of the β-amyloid peptide (Aβ) and neurofibrillary tangles (NFTs) which are composed of a highly-phosphorylated form of the microtubule-associated protein tau. Based on the above hypothesis, there are currently no sufficiently effective technologies and drugs for early detection and treatment of AD. Even the most promising new drug Lecanemab that is based on an anti-amyloid monoclonal antibody therapy, has only partially slowed down the cognitive performance of patients with mild impairment caused by Alzheimer's disease. The main symptoms of AD brain tissue lesions in patients are the deposition of β-amyloid peptide and the hyperphosphorylation of tau protein, which aggregates the microtubule structure of neurons. Therefore, Aβ deposition and hyperphosphorylation of Tau are important pathological biomarkers of Alzheimer's disease. Therefore, the main targets of research for AD prevention, detection and pharmaceuticals are still Aβ and Tau protein. The aim of this study was to detect the changes of Aβ and Tau proteins in the mouse brain tissue with AD and control samples using Visible Resonance Raman (VRR) spectroscopic technology. An attempt was made to develop criteria for the detection of early AD lesions by optical spectroscopy technology. The VRR spectra of AD, the control mouse brain tissues, and Aβ and Tau proteins were recorded and analyzed. The AD and the control mouse brain tissue samples were selected from the thalamus, frontal lobe cortex and hippocampus brain areas. VRR technology with high spatial resolution and the resonance-enhanced features of certain protein molecules is first used in this study to detect and characterize the changes of Aβ and Tau proteins in AD mouse brain model. The optical spectroscopy biomarkers of AD and Control brain tissue were identified in fingerprint and the high-wavenumber regions. The Raman spectra of the secondary structure of protein in amide (I-II-III-B-A) are detected and analyzed. The results indicate that the intensity of Amide I decreased at the 1666 cm^-1 corresponding to the β-sheet structure, and the intensity of the amide III bands (1220- 1320 cm^-1) increased in all AD brain tissues. It was also observed that the Raman peaks of 1448 and 980 cm^-1 related to the abundance of proline, serine, and threonine at tau phosphorylation sites were significantly enhanced in the frontal lobe cortex and hippocampus of AD brain tissues. The intensity ratio biomarker of high phosphorylation in the high wavenumber range from 2898 to 2932 cm-1 increased in all AD brain tissues. Changes of protein secondary conformation and abnormally phosphorylated tau or tauopathies were observed. In summary, VRR is a sensitive tool for characterizing protein structural changes and monitoring the tau phosphorylation. It may potentially be used for early detection of AD.

Proceedings Article | 11 March 2015 Paper

Propagation and transmission of optical vortex beams through turbid scattering wall with orbital angular momentums

W. Wang, Richard Gozali, Thien An Nguyen, R. Alfano

Proceedings Volume 9318, 931805 (2015) https://doi.org/10.1117/12.2186454

KEYWORDS: Particles, Laser scattering, Scattering, Scattering media, Light scattering, Gaussian beams, Optical vortices, Latex, Geometrical optics, Light wave propagation

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Proceedings Article | 11 March 2015 Paper

Tryptophan as key biomarker to detect gastrointestinal tract cancer using non-negative biochemical analysis of native fluorescence and Stokes Shift spectroscopy

Leana Wang, Yan Zhou, Cheng-hui Liu, Lixin Zhou, Yong He, Yang Pu, Thien An Nguyen, Robert Alfano

Proceedings Volume 9318, 93180X (2015) https://doi.org/10.1117/12.2077304

KEYWORDS: Tissues, Luminescence, Cancer, Colorectal cancer, Spectroscopy, Colon, Collagen, Biological research, Fluorescence spectroscopy, Rectum

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Proceedings Article | 25 February 2014 Paper

Incoherent polarized white-light vecctor vortex from a q-plate

Thien An Nguyen, Yisa Rumala, Giovanni Milione, Daniel Nolan, Ebrahim Karimi, Sergei Slussarenko, Lorenzo Marrucci, Robert Alfano

Proceedings Volume 8999, 89990P (2014) https://doi.org/10.1117/12.2042111

KEYWORDS: Optical vortices, Polarization, Lamps, Spiral phase plates, Liquid crystals, Light wave propagation, Linear polarizers, Beam propagation method, Optical filters, Wave plates

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Proceedings Article | 25 February 2014 Paper

Tuning vector vortex in spatially coherent supercontinuum multicolored optical beam using q-plate

Yisa Rumala, Giovanni Milione, Thien An Nguyen, Sebastião Pratavieira, Zabir Hossain, Daniel Nolan, Sergei Slussarenko, Ebrahim Karimi, Lorenzo Marrucci, Robert Alfano

Proceedings Volume 8999, 89990D (2014) https://doi.org/10.1117/12.2042102

KEYWORDS: Polarization, Optical vortices, Source mask optimization, Multiplexing, Spiral phase plates, Optical filters, Channel projecting optics, Charge-coupled devices, Liquid crystals, Ocean optics

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SPIE Journal Paper | 6 January 2014 Open Access

Spatial frequency analysis of anisotropic drug transport in tumor samples

Stewart Russell, Kimberley Samkoe, Jason Gunn, P. Jack Hoopes, Thienan Nguyen, Milo Russell, Robert Alfano, Brian Pogue

JBO, Vol. 19, Issue 01, 015005, (January 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.1.015005

KEYWORDS: Tumors, Spatial frequencies, Tissues, Biological research, Statistical analysis, Anisotropy, Solids, Fourier transforms, Image segmentation, Nanoparticles

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SPIE Journal Paper | 3 January 2014 Open Access

Spatial frequency analysis of high-density lipoprotein and iron-oxide nanoparticle transmission electron microscope image structure for pattern recognition in heterogeneous fields

Stewart Russell, Thien An Nguyen, Stephen Bhagroo, Clyde Torres, Milo Russell, Robert Alfano

JBO, Vol. 19, Issue 01, 015004, (January 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.1.015004

KEYWORDS: Spatial frequencies, Nanoparticles, Particles, Tumors, Fourier transforms, Image transmission, Transmission electron microscopy, Electron microscopes, Biological research, Image analysis

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