Supercontinuum (SC) lasers in combination with tunable acousto-optic filters facilitate multiwavelength digital holographic microscopy (DHM) with a broad spectral range. However, to achieve optimized quantitative phase imaging (QPI) in off-axis DHM the coherence properties of the tunable light source and the holographic carrier interference fringe pattern formation have to be considered as they are wavelength dependent. We thus determined experimentally the wavelength dependency of coherence length and off-axis carrier fringe frequency in a Michelson interferometer-based multi-spectral DHM setup with a continuously tunable SC laser and moreover analyzed the impact of both parameters on hologram formation and QPI image quality. The results are found in good agreement with theoretical predictions and provide essential information for a reliable adjustment of SC laser based multispectral off-axis DHM systems. Finally, the application of multispectral-DHM for reduction of coherence induced disturbances in quantitative phase images of living pancreatic tumor cells in illustrated.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.