Objective: to study the dynamics of the state of autografts of skin and allodermal protectors on a wound using multimodal optical monitoring. Material and methods. A burn wound was simulated in rats (n = 16), 20% of the wound area was covered with skin autografts. The allodermal protector of 0.35 mm thick was applied over the autografts. Studied in vivo the state of the grafts for 10 days: saturation - according to diffuse optical spectroscopy (DOS); perfusion - according to laser Doppler flowmetry (LDF); microstructure - according to optical coherence tomography (OCT). Results. Multimodal monitoring of blood circulation, metabolism and microstructure of skin grafts on a burn wound showed that changes in auto- and allografts occur asynchronously. In the tissues of the autograft, blood saturation directly correlated with the restoration of perfusion (Spearman's coefficient = 0.795); in the allograft, the correlation between perfusion and saturation was weakly inverse (-0.179). Those differences were confirmed by OCT data and histological analysis: allografts lost their normal microstructure simultaneously with a rapid decrease of the blood saturation, despite the preservation of perfusion parameters.
The objective of the research was a multifaceted study of strangulated small intestine to reveal the optical, morphofunctional and biochemical signs of small bowel ischemia. The study was carried out in vivo using an artificially induced strangulation model of the small intestine (together with its mesentery and blood vessels) in 12 Wistar rats. Over a period of 120 minutes following the bowel ligation, changes in the density of the intramural vasculature and intestinal wall microstructure were detected using multimodal optical coherence tomography (MM OCT). Fluorescence lifetime changes of endogenous fluorophores were also measured using macro-FLIM of the strangulated loop and the adjacent segments of the intestine. At the end of the experiment, a morphometric study of the thickness of the layers and the prevalence of necrosis in the intestinal wall was carried out. A comprehensive analysis of the results of the OCT, FLIM and morphometry of the ischemic wall of the small intestine made it possible to determine the correlating morphofunctional and biochemical manifestations that are specific to this model of mesenteric blood flow disturbance.
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