In modern intelligent living, Carbon dots exhibit the great potential by virtue of their unique structure and attractive optical performance serving a range of monitoring and management, from biochemical analysis, biological imaging and environmental monitoring. In this paper, a sub-micro line spacing standard shape was designed based on phosphorescence carbon dots. This spacing standard not only has an unequally spacing line widths, but also shows that the particle size distribution of Carbon dots range from 1.0nm to 10nm. On the other hand, in order to exhibit the potential of our designed shape, a series of experiments were preformed to demonstrate the relationship between fluorescence intensity and special effects. Those results have been proven to be amenable for practical purposes through many tests so that it might be applicable to achieve lighting illumination in different scenes.
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