Mr. Xiaohan Du Profile

Xiaohan Du

at Beijing Information Science & Technology Univ.

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 19 December 2022 Poster + Paper

High-throughput and real-time cell contraction monitoring based on optical Fourier transform

Wenqiang Fan, Fan Zhang, Hongmei Xu, Xiaohan Du, Guanqi Zhao, Jiang Zhu

Proceedings Volume 12320, 123201F (2022) https://doi.org/10.1117/12.2642183

KEYWORDS: Magnetism, Fourier transforms, Imaging systems, Optical filters, Glasses, Spatial filters, Photoresist materials, Particles, Objectives, Image visualization

Read Abstract +

Forces generated by cells are critical regulators of cell adhesion and function. Polymeric micropillar arrays have been widely used for measuring cellular traction forces, however, the sub-micron scale deflections of micropillars are typically measured with a high power microscope, leading to a limited field-of-view and reducing the measurement efficiency. Herein, we reports a typical 4F system used to monitor cell contraction force based on optical Fourier transform (OFT). Compared to conventional microscopy, with a field-of-view on the scale of millimeters and instant acquisition of the force map, this method makes high-throughput and real-time cell contraction monitoring possible. The cells growing on micropillar arrays were placed on the object plane of a typical 4F system, illuminated by a monochromatic plane wave. OFT was performed on the light field as it passed through the first Fourier lens. The spatial spectrum that consists of discrete light spots was projected on the back focal plane of the first Fourier lens and was then filtered by an optical stop, eliminating the extraneous frequency components while allowing the frequency spots corresponding to the period of the micropillar arrays to pass through the aperture. Inverse OFT was conducted as the filtered light passed through the second Fourier lens and an image was reconstructed on the image plane of the 4F system, which was recorded by a CCD camera with a 20X objective. The light intensity of the image directly represents the degree of periodicity of the micropillar arrays, in which low-intensity areas indicated that the micropillars in this area were deflected while high-intensity areas indicate the presence of uniform micropillar arrays. Using this method, cell contraction forces could be directly visualized based on the intensity distribution at the image plane with no need for further image post-processing, enabling direct visualization of cell contraction in a larger field-of-view.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print or electronic format on SPIE.org.

ORGANIZATIONAL
Sign in with credentials provided by your organization.

Organizational Username

Organizational Password

Show/Hide Password

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years