Mr. Yichen Feng Profile

Yichen Feng

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Publications (4)

Proceedings Article | 13 March 2024 Presentation + Paper

Quantitative tissue biodistribution of fluorescence imaging agents in orthotopic mouse model of head and neck squamous cell carcinoma

Yichen Feng, Cody Rounds, Kenneth Tichauer, Kimberley Samkoe

Proceedings Volume 12821, 1282108 (2024) https://doi.org/10.1117/12.3002872

KEYWORDS: Tissues, Fluorescence imaging, Tumors, Biological imaging, Fluorescence, Fluorescence intensity

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Proceedings Article | 13 March 2024 Poster + Paper

Time dependent quantification of paired agent imaging in head and neck squamous cell carcinomas: determining the effects of imaging agent concentrations on binding potential

Sanjana Pannem, Yichen Feng, Sassan Hodge, Eunice Chen, Jonathan Elliott, Kenneth Tichauer, Keith Paulsen, Kimberley Samkoe

Proceedings Volume 12825, 128250C (2024) https://doi.org/10.1117/12.3002812

KEYWORDS: Fluorescence, Fluorescence intensity, Fluorescence imaging, Dyes, Neck, Head, Tissues, Biological imaging, Visualization, Tumor growth modeling

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SPIE Journal Paper | 17 January 2024 Open Access

Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment

Arthur F. Petusseau, Samuel S. Streeter, Arin Ulku, Yichen Feng, Kimberley S. Samkoe, Claudio Bruschini, Edoardo Charbon, Brian W. Pogue, Petr Bruza

JBO, Vol. 29, Issue 01, 016004, (January 2024) https://doi.org/10.1117/12.10.1117/1.JBO.29.1.016004

KEYWORDS: Fluorescence, Tissues, Reflectivity, Optical properties, Single photon avalanche diodes, Fluorescence intensity, Calibration, Tumors, Time of flight imaging, Gelatin

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SignificanceFluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecular phenotypic information about the disease being resected. Conventional fluorescence-guided surgery relies on long, microsecond scale laser pulses to excite fluorescent probes. However, this technique only provides two-dimensional information; crucial depth information, such as the location of malignancy below the tissue surface, is not provided.AimWe developed a depth sensing imaging technique using light detection and ranging (LiDAR) time-of-flight (TOF) technology to sense the depth of target tissue while overcoming the influence of tissue optical properties and fluorescent probe concentration.ApproachThe technology is based on a large-format (512×512 pixel), binary, gated, single-photon avalanche diode (SPAD) sensor with an 18 ps time-gate step, synchronized with a picosecond pulsed laser. The fast response of the sensor was developed and tested for its ability to quantify fluorescent inclusions at depth and optical properties in tissue-like phantoms through analytical model fitting of the fast temporal remission data.ResultsAfter calibration and algorithmic extraction of the data, the SPAD LiDAR technique allowed for sub-mm resolution depth sensing of fluorescent inclusions embedded in tissue-like phantoms, up to a maximum of 5 mm in depth. The approach provides robust depth sensing even in the presence of variable tissue optical properties and separates the effects of fluorescence depth from absorption and scattering variations.ConclusionsLiDAR TOF fluorescence imaging using an SPAD camera provides both fluorescence intensity images and the temporal profile of fluorescence, which can be used to determine the depth at which the signal is emitted over a wide field of view. The proposed tool enables fluorescence imaging at a higher depth in tissue and with higher spatial precision than standard, steady-state fluorescence imaging tools, such as intensity-based near-infrared fluorescence imaging, optical coherence tomography, Raman spectroscopy, or confocal microscopy. Integration of this technique into a standard surgical tool could enable rapid, more accurate estimation of resection boundaries, thereby improving the surgeon’s efficacy and efficiency, and ultimately improving patient outcomes.

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Proceedings Article | 14 March 2023 Presentation + Paper

Development of near-infrared paired-agent imaging for in vivo quantification of drug receptor occupancy in tumor

Yichen Feng, Kenneth Tichauer, Kimberley Samkoe

Proceedings Volume 12357, 1235709 (2023) https://doi.org/10.1117/12.2650968

KEYWORDS: Tumors, In vivo imaging, Fluorescence, Animals, Tissues, Target acquisition, Reflection, Molecules, Fluorescence imaging, Muscles

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