Localized surface plasmon was formed with plasmonic nanohole arrays for measurement of the Raman spectrum in polystyrene beads. For the selective acquisition of enhanced Raman signals, we used the surface-enhanced Raman spectroscopy (SERS) system employing particle manipulation within the electrode chip. This integration involves the amalgamation of metal nanostructures and microelectrodes to assess the electrophysiological characteristics of microparticles. The manipulation of particle movement, contingent upon their electrical properties such as conductivity and permittivity, can be controlled through modulation of the frequency of the applied alternating current field. In our integrated system, Raman spectroscopy can be conducted with enhanced intensity. Real-time measurement of enhanced Raman scattering is achieved by controlling the direction of force exerted on single particle and selectively trapping it on the nanostructured surfaces.
When a microparticle is exposed to an alternating current (AC) electric field, the polarized particle is moved by the interaction between the dipoles and the AC electric field under dielectrophoresis (DEP). The DEP force is widely used for manipulation of microparticles in diverse practical applications such as 3D manipulation, sorting, transfer, and separation of various particles such as living cells. In this study, we propose integration of surface-enhanced Raman spectroscopy (SERS), an extremely sensitive and versatile technique based on Raman scattering of molecules supported by nanostructured materials, with DEP using a microfluidic device, i.e., the microfluidic device combines metal nanostructures and microelectrodes to characterize electrophysiological and biochemical properties of biological cells. The movement of particles, which varies depending on the electrical properties such as conductivity and permittivity of particles, can be manipulated by the cross-frequency change, which is one of the DEP properties. Raman spectroscopy using this DEP-SERS integrated system can be performed with an improved signal-to-noise ratio by determining the direction of the DEP force applied to the cells with respect to the applied AC power and collecting them on the nanostructure.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.