A tunable acoustic gradient lens and a resonant mirror are integrated into a multiphoton excited microscope that is rapidly and precisely controlled by an embedded field programmable gate array to acquire images at 8 kHz for x-z plane. The pulse train signal of a femtosecond laser provides up to 95 MHz voxel rate, and one pulse per pixel. A volumetric image rate at up to 30 Hz can be realized according to the precise focal position and the excited signal, and deep learning. Biological images can be demonstrated at high-speed volumetric imaging rate.
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