In this work, we compare the performance of a quantitative scientific complementary metal-oxide semiconductor
(qCMOS) camera to the sCMOS camera for multiphoton imaging of tissue specimens. We find that the qCMOS
achieves a signal-to-background ratio that is ~2x and ~1.6x higher than that achieved by the sCMOS for twophoton
fluorescence and second-harmonic generation (SHG) imaging, respectively. The field-of-view of the
qCMOS camera is noticeably larger at ~1.3x that of the sCMOS. We also confirm that the qCMOS can spatially
resolve features as fine as 12.5 μm in 200-μm thick tendon tissue, at a penetration depth of 140 μm, using SHG
imaging. Our results highlight the applicability of the qCMOS for some multiphoton imaging applications.
In this work, we study the self-healing properties of the space-time (ST) light sheet upon interaction with a partially transparent object, namely a microtissue array of biological samples. In particular, we study the response of the ST light sheet upon encountering biological tissues that have been graded as normal (benign), malignant, and malignant tumor stage III. We find a differential response that can be leveraged for characterization of the tissue specimens. Specifically, the ST light sheet is observed to more completely reconstruct upon traversing the normal tissue compared to the malignant specimens. We believe that this is the first report exploring the potential of self-healing as a metric for tissue characterization.
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