For decades the need for organs for transplantation by far exceeds their availability, costing the lives of patients in desperate need for a transplant. This need has acted as driving force for the growth of the tissue engineering field. For the most part, tissue engineering utilizes scaffolds made of decellularized tissues or synthetic and natural hydrogels that can be polymerized. More recently, bioprinting has emerged as a robust alternative for precise placing of cells onto scaffolds or into polymers. Laser bioprinting, in particular, allows high speed and precise printing of cells into any bioink and with high viability. In this study, we use laser bioprinting to engineer urinary bladder tissue for regenerative medicine applications. Using a natural hydrogel and primary smooth muscle cells and urothelial cells, we have managed to print ex vivo bladder explants which recapitulate the structure of native urinary bladder.
The use of different 3D printing technologies for pharmaceutical manufacturing provides new opportunities for personalized medicine with adjusted doses in various shapes and structures. In this work, we present a novel manufacturing method of printing personalized dosage forms onto edible wafer papers as orodispersible thin films, targeting diseases such as types of cancer. Orodispersible films (ODFs) hold promise as a novel drug delivery method as they are easy to administer to young and older patients, bypass absorption by the digestive system and minimize the risk of partial loss of actives due to table crashing or imprecise liquid administration. LIFT technology could be used to “print” paclitaxel molecules on various surfaces that could be used as depot formulations. Laser-Induced Forward Transfer (LIFT) has been successfully applied in the nanosecond regime for the controlled transfer of paclitaxel solution onto the receiving substrates. Quantification studies of printed paclitaxel amount were determined by means of a Mass Spectrometry (MS)-based analytical technique. Furthermore, initial studies were performed where the printed paclitaxel wafer papers were used as orodispersible films (ODFs) on mice for oral mucosal drug delivery application, revealing encouraging results.
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