Matrix metalloproteinase 9 (MMP9) is present in normal physiological events in the human body. However, it is also known for being involved in inflammation process related to different diseases, such as arthritis and metastasis. Thus, this protein can be used as a biomarker for diseases monitoring and therapies control and assessments. The goal of this study is to demonstrate that novel multiplexed R-NPs optical transducers independent arrays, (also called BICELLs, Biological Sensing Cells) can discriminate small concentrations of MMP9. We achieved a LoD of 84 ng/mL. This data can be improved by increasing the resolution of the characterization system and optimizing the biofunctionalization strategy. These results are promising and encourage us to perform prospective works addressed to replicate this experiment using a fluidic system for multiplexed real-time optical response, so as to facilitate the monitoring of the different selected biomarkers (thank to multiplexed configuration of R-NPS arrays), necessary to provide patients with a more accurate diagnosis and treatments, and therefore to boost up personalized medicine.
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