Using dual-modality imaging, we can detect with this system the molecular composition of tissues on a 1 cm² area. Visible light imaging is done in real-time through an RGB camera, while the Raman modality uses a line-scanning system for surface imaging and has a spatial resolution of 250 μm² as well as a spectral resolution of 8 cm-1 for measurements just under 10 seconds. This system is used for ex-vivo measurements, where in a recent study, this system differentiated invasive breast cancer from normal breast tissues based on an SVM classification model.
We present a macroscopic line scanning Raman imaging system which has been modified to be suitable for intraoperative use. A sterilizable probe muzzle was designed to flatten the biological tissue ensuring its position at the focal plane of the Raman probe optics, removing the need for probe sterilization. The system uses a flexible imaging probe with a 1cm2 field of view to record fingerprint Raman images, mounted on an articulated arm that supports the probe weight and allows gentle contact with the tissue. Validation results obtained on porcine tissues show >95% classification accuracy between different tissue types.
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