We propose a label-free metrology technique that can perform analysis of live individual cells while they are growing individually in droplets. The cells first flow in a microfluidics device and imaged using interferometry. At the end of this device the droplets with the cells accumulate and then the cells can be monitored over time. The device presents the possibility for both high-throughput label-free analysis of individual biological cells and tracking their long-term development.
We propose a label-free technique that performs analysis of live individual cells during flow and then sort the cells based on the analysis results. The cells rapidly flow in a droplet microfluidics device, which prevents two cells in the field of view in once. By acquiring holograms of each cell during flow and achieving the cell optical path delay (OPD) profile, we extract different features that differentiate between the cells, based on which rapid sorting is performed. The device presents the possibility for both high-throughput label-free analysis of individual cells and their real-time sorting.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.