Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

Syam Mohan P.C. Mohanan; Bethany Mills; Kay Russell; Sheelagh Duncan; Alex Kiang; Rachel L. Williams; Venkatesh Prajna; Kevin Dhaliwal M.D.; Gareth O. S. Williams

doi:10.1117/12.2609578

7 March 2022 Frugal fluorescence imaging of microbes: proof of concept for translation to point of care

Syam Mohan P.C. Mohanan, Bethany Mills, Kay Russell, Sheelagh Duncan, Alex Kiang, Rachel L. Williams, Venkatesh Prajna, Kevin Dhaliwal M.D., Gareth O. S. Williams

Author Affiliations +

Proceedings Volume PC11950, Optics and Biophotonics in Low-Resource Settings VIII; PC1195005 (2022) https://doi.org/10.1117/12.2609578
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Fluorescence imaging and ‘smart probes’ are an emerging point-of-care platform for microbial detection. This article proposes a proof-of-concept frugal fluorescence imaging system for the detection of bacteria such as Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. In this work, we investigate the capability of a trans-illuminating fluorescence imaging system to detect bacteria using a low-cost raspberry pi single board computer and a high-quality camera. This system is capable of producing high quality fluorescence and transmission images of bacterial samples with submicron resolution. We demonstrate the capabilities of the system to produce these images in conjunction with low-cost lens-based imaging optics.

Conference Presentation

Citation Download Citation

Syam Mohan P.C. Mohanan, Bethany Mills, Kay Russell, Sheelagh Duncan, Alex Kiang, Rachel L. Williams, Venkatesh Prajna, Kevin Dhaliwal M.D., and Gareth O. S. Williams "Frugal fluorescence imaging of microbes: proof of concept for translation to point of care", Proc. SPIE PC11950, Optics and Biophotonics in Low-Resource Settings VIII, PC1195005 (7 March 2022); https://doi.org/10.1117/12.2609578

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