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7 March 2022 DO-SRS imaging of metabolic dynamics regulated by aromatic amino acids
Lingyan Shi, Pegah Bagheri, Khang Hoang, Anthony Fung, Sahran Hussain
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Proceedings Volume PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry; PC119570T (2022) https://doi.org/10.1117/12.2615575
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
We use D2O probed stimulated Raman scattering (DO-SRS) and Multiphoton Fluorescence (MPF) microscopy to visualize metabolic changes in HeLa cells under excess AAA of phenylalanine or tryptophan. The cellular spatial distribution of de novo lipogenesis, protein synthesis, NADH, Flavin, unsaturated lipids, and cholesterol were all imaged and quantified in this experiment. Our studies reveal the increase in NADH to Flavin ratio by 10% and unsaturated lipids to saturated by 50% in cells treated with excess phenylalanine and tryptophan. Our study shows that DO-SRS can be used to as a high resolution imaging platform to study AAA regulated metabolic activities in cells.
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Lingyan Shi, Pegah Bagheri, Khang Hoang, Anthony Fung, and Sahran Hussain "DO-SRS imaging of metabolic dynamics regulated by aromatic amino acids", Proc. SPIE PC11957, Biomedical Vibrational Spectroscopy 2022: Advances in Research and Industry, PC119570T (7 March 2022); https://doi.org/10.1117/12.2615575
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KEYWORDS
Multiphoton fluorescence microscopy
Proteins
Cancer
Diseases and disorders
Image resolution
Microscopy
Multiphoton microscopy
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