Exchangeable fluorophore labels in super-resolution fluorescence microscopy

Mike Heilemann

doi:10.1117/12.2612522

2 March 2022 Exchangeable fluorophore labels in super-resolution fluorescence microscopy

Mike Heilemann

Proceedings Volume PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV; PC119670C (2022) https://doi.org/10.1117/12.2612522
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present fluorophore labels that transiently and repetitively bind to their targets as probes for various types of super-resolution fluorescence microscopy. Such labels show a weak (~10 µM – 100 nM) affinity to a target and are kept in an imaging buffer that constitutes a reservoir with a high concentration of intact probes, enabling repetitive binding to the same target (we refer to these labels as “exchangeable labels”). This dynamic labeling approach minimizes photobleaching and yields a constant fluorescence signal over time, which has been beneficially exploited in SMLM [1-4], STED [5, 6], and super-resolution optical fluctuation imaging (SOFI) [7]. Multi-color, 3D, and live cell imaging, as well as imaging of large fields of view, is facilitated [4]. We further present the implementation of neural networks for multi-emitter localization to achieve multi-color SMLM with short acquisition times of one minute [8].

Conference Presentation

Citation Download Citation

Mike Heilemann "Exchangeable fluorophore labels in super-resolution fluorescence microscopy", Proc. SPIE PC11967, Single Molecule Spectroscopy and Superresolution Imaging XV, PC119670C (2 March 2022); https://doi.org/10.1117/12.2612522

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available