CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC11973 > Article
Presentation
2 March 2022 Enhanced tri-modal Optical-Photothermal Infrared (O-PTIR) spectroscopy – advances in spatial resolution, sensitivity & tri-modality (IR, Raman & Fluorescence)
Mustafa Kansiz, Craig Prater, Jay Anderson
Author Affiliations +
Proceedings Volume PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022; PC119730O (2022) https://doi.org/10.1117/12.2615403
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Optical Photothermal Infrared (O-PTIR) spectroscopy has established itself as a cutting edge vibrational microspectroscopy tool, offering significant advantages over traditional FTIR/QCL and Raman spectroscopic tools, providing submicron simultaneous IR+Raman microscopy, in non-contact mode with high sensitivity. A new modality, “counter-propagating” has been engineered to provide for enhanced IR (and Raman) spatial resolution and sensitivity, with the use of high NA refractive objectives. This improves spatial resolution to ~300nm for both IR and Raman, whilst also improving sensitivity, image quality and facilitating immersion objective studies in fluid. Further, we coupled a widefield epifluorescence to facilitate a novel concept – fluorescence guided (or fluorescence co-located) O-PTIR microspectroscopy. Several life sciences examples from bacteria, cells and tissues will be provided to demonstrate these capabilities and how they can enable new experiments and research findings.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Mustafa Kansiz, Craig Prater, and Jay Anderson "Enhanced tri-modal Optical-Photothermal Infrared (O-PTIR) spectroscopy – advances in spatial resolution, sensitivity & tri-modality (IR, Raman & Fluorescence)", Proc. SPIE PC11973, Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, PC119730O (2 March 2022); https://doi.org/10.1117/12.2615403
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Infrared spectroscopy
Raman spectroscopy
Luminescence
Spatial resolution
Infrared radiation
Spectroscopy
Life sciences
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top